TY - JOUR
T1 - LC/MS/MS analysis of leukotriene B4 in exhaled breath condensate for assessing lung inflammation.
AU - Montuschi, Paolo
PY - 2009
Y1 - 2009
N2 - Leukotriene (LT) B(4) is a potent inflammatory lipid mediator that has been involved in the pathophysiology of respiratory diseases including asthma. Exhaled breath condensate (EBC) is a non-invasive method to sample secretions from the airways. LC/MS/MS techniques for measuring LTB(4) concentrations in EBC have been developed and are suitable for an accurate quantitative assessment of its concentrations in EBC. LC/MS/MS for other eicosanoids including 8-isoprostane, a marker of oxidative stress, and cysteinyl-LTs have been developed. This article, mainly focused on LTB(4), presents the analytical aspects of the LC/MS/MS techniques for measuring LTB(4) and 8-isoprostane in EBC, provides examples of their application to the assessment of airway inflammation in patients with asthma and other respiratory diseases, and discusses their potential utility for non-invasive monitoring of drug therapy.
AB - Leukotriene (LT) B(4) is a potent inflammatory lipid mediator that has been involved in the pathophysiology of respiratory diseases including asthma. Exhaled breath condensate (EBC) is a non-invasive method to sample secretions from the airways. LC/MS/MS techniques for measuring LTB(4) concentrations in EBC have been developed and are suitable for an accurate quantitative assessment of its concentrations in EBC. LC/MS/MS for other eicosanoids including 8-isoprostane, a marker of oxidative stress, and cysteinyl-LTs have been developed. This article, mainly focused on LTB(4), presents the analytical aspects of the LC/MS/MS techniques for measuring LTB(4) and 8-isoprostane in EBC, provides examples of their application to the assessment of airway inflammation in patients with asthma and other respiratory diseases, and discusses their potential utility for non-invasive monitoring of drug therapy.
KW - LC/MS
KW - condensato del respiro
KW - infiammazione polmonare
KW - leucotriene B4
KW - LC/MS
KW - condensato del respiro
KW - infiammazione polmonare
KW - leucotriene B4
UR - https://publicatt.unicatt.it/handle/10807/31061
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=64149084268&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=64149084268&origin=inward
U2 - 10.1016/j.jchromb.2009.01.036
DO - 10.1016/j.jchromb.2009.01.036
M3 - Article
SN - 1570-0232
VL - 877
SP - 1272
EP - 1280
JO - JOURNAL OF CHROMATOGRAPHY. B
JF - JOURNAL OF CHROMATOGRAPHY. B
IS - Maggio
ER -