LC/MS/MS analysis of leukotriene B4 in exhaled breath condensate for assessing lung inflammation.

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Abstract

Leukotriene (LT) B(4) is a potent inflammatory lipid mediator that has been involved in the pathophysiology of respiratory diseases including asthma. Exhaled breath condensate (EBC) is a non-invasive method to sample secretions from the airways. LC/MS/MS techniques for measuring LTB(4) concentrations in EBC have been developed and are suitable for an accurate quantitative assessment of its concentrations in EBC. LC/MS/MS for other eicosanoids including 8-isoprostane, a marker of oxidative stress, and cysteinyl-LTs have been developed. This article, mainly focused on LTB(4), presents the analytical aspects of the LC/MS/MS techniques for measuring LTB(4) and 8-isoprostane in EBC, provides examples of their application to the assessment of airway inflammation in patients with asthma and other respiratory diseases, and discusses their potential utility for non-invasive monitoring of drug therapy.
Original languageEnglish
Pages (from-to)1272-1280
Number of pages9
JournalJOURNAL OF CHROMATOGRAPHY. B
Volume877
DOIs
Publication statusPublished - 2009

Keywords

  • LC/MS
  • condensato del respiro
  • infiammazione polmonare
  • leucotriene B4

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