Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes

Diego Voccia, D. Voccia, M. Sosnowska, F. Bettazzi, I. Palchetti, W. Kutner

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.
Original languageEnglish
Title of host publicationLecture Notes in Electrical Engineering
Pages59-64
Number of pages6
Volume319
DOIs
Publication statusPublished - 2015
Event2nd National Conference on Sensors, 2014 - ita
Duration: 19 Feb 201421 Feb 2014

Publication series

NameLECTURE NOTES IN ELECTRICAL ENGINEERING

Conference

Conference2nd National Conference on Sensors, 2014
Cityita
Period19/2/1421/2/14

Keywords

  • Electropolymerization

Fingerprint

Dive into the research topics of 'Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes'. Together they form a unique fingerprint.

Cite this