Jejunal proteins secreted by db/db mice or insulin-resistant humans impair the insulin signaling and determine insulin resistance

Serenella Salinari; Cyrille Debard; Alessandro Bertuzzi; Christine Durand; Paul Zimmet; Hubert Vidal; Geltrude Mingrone

doi:10.1371/journal.pone.0056258

Jejunal proteins secreted by db/db mice or insulin-resistant humans impair the insulin signaling and determine insulin resistance

Serenella Salinari, Cyrille Debard, Alessandro Bertuzzi, Christine Durand, Paul Zimmet, Hubert Vidal, Geltrude Mingrone

Research output: Contribution to journal › Article

41 Citations (Scopus)

Abstract

Two recent studies demonstrated that bariatric surgery induced remission of type 2 diabetes very soon after surgery and far too early to be attributed to weight loss. In this study, we sought to explore the mechanism/s of this phenomenon by testing the effects of proteins from the duodenum-jejunum conditioned-medium (CM) of db/db or Swiss mice on glucose uptake in vivo in Swiss mice and in vitro in both Swiss mice soleus and L6 cells. We studied the effect of sera and CM proteins from insulin resistant (IR) and insulin-sensitive subjects on insulin signaling in human myoblasts.

Original language	English
Pages (from-to)	e56258-e56258
Journal	PLoS One
Volume	8
DOIs	https://doi.org/10.1371/journal.pone.0056258
Publication status	Published - 2013

Keywords

Adult
Animals
Cell Differentiation
Culture Media, Conditioned
Deoxyglucose
Diabetes Mellitus, Experimental
Glycogen Synthase Kinase 3
Humans
Insulin
Insulin Resistance
Jejunum
Mice
Mice, Inbred C57BL
Muscle Fibers, Skeletal
Phosphorylation
Phosphoserine
Proteins
Proto-Oncogene Proteins c-akt
Ribosomal Protein S6 Kinases, 70-kDa
Signal Transduction

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1371/journal.pone.0056258

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title = "Jejunal proteins secreted by db/db mice or insulin-resistant humans impair the insulin signaling and determine insulin resistance",

abstract = "Two recent studies demonstrated that bariatric surgery induced remission of type 2 diabetes very soon after surgery and far too early to be attributed to weight loss. In this study, we sought to explore the mechanism/s of this phenomenon by testing the effects of proteins from the duodenum-jejunum conditioned-medium (CM) of db/db or Swiss mice on glucose uptake in vivo in Swiss mice and in vitro in both Swiss mice soleus and L6 cells. We studied the effect of sera and CM proteins from insulin resistant (IR) and insulin-sensitive subjects on insulin signaling in human myoblasts.",

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author = "Serenella Salinari and Cyrille Debard and Alessandro Bertuzzi and Christine Durand and Paul Zimmet and Hubert Vidal and Geltrude Mingrone",

year = "2013",

doi = "10.1371/journal.pone.0056258",

language = "English",

volume = "8",

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journal = "PLoS One",

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T1 - Jejunal proteins secreted by db/db mice or insulin-resistant humans impair the insulin signaling and determine insulin resistance

AU - Salinari, Serenella

AU - Debard, Cyrille

AU - Bertuzzi, Alessandro

AU - Durand, Christine

AU - Zimmet, Paul

AU - Vidal, Hubert

AU - Mingrone, Geltrude

PY - 2013

Y1 - 2013

N2 - Two recent studies demonstrated that bariatric surgery induced remission of type 2 diabetes very soon after surgery and far too early to be attributed to weight loss. In this study, we sought to explore the mechanism/s of this phenomenon by testing the effects of proteins from the duodenum-jejunum conditioned-medium (CM) of db/db or Swiss mice on glucose uptake in vivo in Swiss mice and in vitro in both Swiss mice soleus and L6 cells. We studied the effect of sera and CM proteins from insulin resistant (IR) and insulin-sensitive subjects on insulin signaling in human myoblasts.

AB - Two recent studies demonstrated that bariatric surgery induced remission of type 2 diabetes very soon after surgery and far too early to be attributed to weight loss. In this study, we sought to explore the mechanism/s of this phenomenon by testing the effects of proteins from the duodenum-jejunum conditioned-medium (CM) of db/db or Swiss mice on glucose uptake in vivo in Swiss mice and in vitro in both Swiss mice soleus and L6 cells. We studied the effect of sera and CM proteins from insulin resistant (IR) and insulin-sensitive subjects on insulin signaling in human myoblasts.

KW - Adult

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KW - Cell Differentiation

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KW - Glycogen Synthase Kinase 3

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KW - Muscle Fibers, Skeletal

KW - Phosphorylation

KW - Phosphoserine

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KW - Proto-Oncogene Proteins c-akt

KW - Ribosomal Protein S6 Kinases, 70-kDa

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KW - Animals

KW - Cell Differentiation

KW - Culture Media, Conditioned

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KW - Insulin

KW - Insulin Resistance

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KW - Mice, Inbred C57BL

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KW - Phosphorylation

KW - Phosphoserine

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KW - Proto-Oncogene Proteins c-akt

KW - Ribosomal Protein S6 Kinases, 70-kDa

KW - Signal Transduction

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M3 - Article

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JO - PLoS One

JF - PLoS One

ER -

Jejunal proteins secreted by db/db mice or insulin-resistant humans impair the insulin signaling and determine insulin resistance

Abstract

Keywords

UN SDGs

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