Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies

Ge Martorana; Elisabetta Meucci Calabrese; Ga Miggiano; Alvaro Mordente; A. Castelli

Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies

Ge Martorana, Elisabetta Meucci Calabrese, Ga Miggiano, Alvaro Mordente, A. Castelli

Faculty of Medicine and Surgery

Research output: Contribution to journal › Article

Abstract

Very low amounts of ascorbic acid modify alkaline phosphatase fluorescence, absorption and enzymatic activity. A strong quenching of enzyme, tryptophan and tyrosine emission together with evident alterations of the protein absorption characteristics are observed. The catalytic activity inhibition probably reflects a perturbation of the active site environment due to the interaction of ascorbic acid with enzyme aminoacyl residues.

Original language	English
Pages (from-to)	231-238
Number of pages	8
Journal	Italian Journal of Biochemistry
Volume	32
Issue number	4
Publication status	Published - 1983

All Science Journal Classification (ASJC) codes

Biochemistry

Keywords

Alkaline Phosphatase
Animals
Ascorbic Acid
Cattle
Fluorescence
Kidney
Spectrometry

Cite this

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title = "Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies",

abstract = "Very low amounts of ascorbic acid modify alkaline phosphatase fluorescence, absorption and enzymatic activity. A strong quenching of enzyme, tryptophan and tyrosine emission together with evident alterations of the protein absorption characteristics are observed. The catalytic activity inhibition probably reflects a perturbation of the active site environment due to the interaction of ascorbic acid with enzyme aminoacyl residues.",

keywords = "Alkaline Phosphatase, Animals, Ascorbic Acid, Cattle, Fluorescence, Kidney, Spectrometry, Alkaline Phosphatase, Animals, Ascorbic Acid, Cattle, Fluorescence, Kidney, Spectrometry",

author = "Ge Martorana and \{Meucci Calabrese\}, Elisabetta and Ga Miggiano and Alvaro Mordente and A. Castelli",

year = "1983",

language = "English",

volume = "32",

pages = "231--238",

journal = "Italian Journal of Biochemistry",

issn = "0021-2938",

publisher = "Biomedia srl",

number = "4",

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TY - JOUR

T1 - Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies

AU - Martorana, Ge

AU - Meucci Calabrese, Elisabetta

AU - Miggiano, Ga

AU - Mordente, Alvaro

AU - Castelli, A.

PY - 1983

Y1 - 1983

N2 - Very low amounts of ascorbic acid modify alkaline phosphatase fluorescence, absorption and enzymatic activity. A strong quenching of enzyme, tryptophan and tyrosine emission together with evident alterations of the protein absorption characteristics are observed. The catalytic activity inhibition probably reflects a perturbation of the active site environment due to the interaction of ascorbic acid with enzyme aminoacyl residues.

AB - Very low amounts of ascorbic acid modify alkaline phosphatase fluorescence, absorption and enzymatic activity. A strong quenching of enzyme, tryptophan and tyrosine emission together with evident alterations of the protein absorption characteristics are observed. The catalytic activity inhibition probably reflects a perturbation of the active site environment due to the interaction of ascorbic acid with enzyme aminoacyl residues.

KW - Alkaline Phosphatase

KW - Animals

KW - Ascorbic Acid

KW - Cattle

KW - Fluorescence

KW - Kidney

KW - Spectrometry

KW - Alkaline Phosphatase

KW - Animals

KW - Ascorbic Acid

KW - Cattle

KW - Fluorescence

KW - Kidney

KW - Spectrometry

UR - https://publicatt.unicatt.it/handle/10807/9756

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=0021016872&origin=inward

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0021016872&origin=inward

M3 - Article

SN - 0021-2938

VL - 32

SP - 231

EP - 238

JO - Italian Journal of Biochemistry

JF - Italian Journal of Biochemistry

IS - 4

ER -

Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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