Inline extracorporeal photopheresis: evaluation of cell collection efficiency

Nicola Piccirillo, Patrizia Chiusolo, Simona Sica, Gina Zini, Rossana Putzulu, Giuseppina Massini, Alessia Di Giovanni

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

BACKGROUND: Extracorporeal photopheresis (ECP) therapy has proved to be an effective and safe treatment for graft-versus-host-disease (GvHD), an important complication after hematopoietic stem cell transplantation. In 2016, we acquired Therakos CellEx, a dedicated inline ECP device to accomplish a significant increase in ECP activity. In literature, we found few data reporting CellEx performance evaluated in terms of collection efficiency to qualify the device. Hence, we decided to collect and analyze our data in order to build a reference in terms of expected results of the procedure. Here we report our data of ECP performed using CellEx in a 12-month period focusing on collection efficiency assessment, as well as procedural and apheretic product characteristics. STUDY DESIGN AND METHODS: We collected data of patients undergoing ECP from April 2018 to March 2019 using CellEx in order to evaluate collection efficiency. RESULTS: Between April 2018 and March 2019 we treated 28 adult patients affected by GvHD performing 319 ECP using CellEx. CellEx mononuclear cell product was characterized by high mononuclear cell percentage and low percentage of granulocytes, resulting particularly suitable for ECP treatments. Median collection efficiency for total nucleated cells and for mononuclear cells was 31.2% and 62.3%, respectively. CONCLUSION: Collection efficiency of CellEx was comparable to that usually obtained by cell separators designed for cell collection and was comparable to that of offline systems. Our results provide a detailed performance evaluation for inline ECP system users.
Original languageEnglish
Pages (from-to)3714-3720
Number of pages7
JournalTransfusion
Volume59
DOIs
Publication statusPublished - 2019

Keywords

  • Adult
  • Aged
  • Erythrocytes
  • Female
  • Graft vs Host Disease
  • Granulocytes
  • Humans
  • Leukocyte Count
  • Linear Models
  • Male
  • Middle Aged
  • Photopheresis

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