Improving impedimetric nucleic acid detection by using enzyme-decorated liposomes and nanostructured screen-printed electrodes

Diego Voccia, Francesca Bettazzi, Emiliano Fratini, Debora Berti, Ilaria Palchetti

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Sensitive impedimetric detection of miR-222, a miRNA sequence found in many lung tumors, was investigated by using gold-nanostructured disposable carbon electrodes and enzyme-decorated liposomes. The proposed method was based on the immobilization of thiolated DNA capture probes onto gold-nanostructured carbon surfaces. Afterwards, the capture probes were allowed to hybridize to the target miRNAs. Finally, enzyme-decorated liposomes were used as labels to amplify the miRNA sensing, by their association with the probe-miRNA hybrids generated on the nanostructured transducer. By using this amplification route a limit of detection of 0.400 pM, a limit of quantification of 1.70 pM, and an assay range spanning three orders of magnitude (1.70-900 pM) were obtained (RSD % = 13). This limit of quantification was 20 times lower than that obtained using a simple enzyme conjugate for the detection. A comparison was also made with gold screen-printed transducers. In this case, a limit of quantification approximately 70 times lower was found by using the nanostructured transducers. Application of the optimized assay in serum samples was also demonstrated.
Original languageEnglish
Pages (from-to)7271-81-7281
JournalAnalytical and Bioanalytical Chemistry
Volume408
DOIs
Publication statusPublished - 2016

Keywords

  • Alkaline Phosphatase
  • Alkaline phosphatase
  • Biosensing Techniques
  • DNA Probes
  • Dielectric Spectroscopy
  • Electrochemical impedance spectroscopy
  • Electrodes
  • Enzymes, Immobilized
  • Gold
  • Gold nanoparticles
  • Humans
  • Immobilized Nucleic Acids
  • Limit of Detection
  • Liposome
  • Liposomes
  • Metal Nanoparticles
  • MicroRNAs
  • Nanostructures
  • Nucleic Acid Hybridization
  • Screen-printed electrodes

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