Identification of PDGF-BB binding to thymosin β4 by chemical cross-linking

Jana Knop; Christine App; Thomas Huff; Federica Iavarone; Massimo Castagnola; Ewald Hannappel

doi:10.1517/14712598.2015.1014793

Identification of PDGF-BB binding to thymosin β4 by chemical cross-linking

Jana Knop
, Christine App
, Thomas Huff
, Federica Iavarone
, Massimo Castagnola
, Ewald Hannappel

Friedrich-Alexander University Erlangen-Nürnberg

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The purpose of our work was to identify unknown interaction partners of thymosin β4 (Tβ4). It was suggested that Tβ4 could be an antifibrotic drug for treatment of liver fibrogenesis, because Tβ4 prevents the platelet-derived growth factor-BB (PDGF-BB)-induced activation of hepatic stellate cells (HSCs). Very little information is available how Tβ4 counteracts the PDGF-BB-induced activation of HSCs. We propose the hypothesis that Tβ4 could bind directly to PDGF-BB and thereby reduce the concentration of free PDGF-BB available for binding to the PDGF-β receptor.

Original language	English
Pages (from-to)	S147-S154
Number of pages	8
Journal	Expert Opinion on Biological Therapy
Volume	15 Suppl 1
DOIs	https://doi.org/10.1517/14712598.2015.1014793
Publication status	Published - 2015

Keywords

biotin label transfer
cross-linking
platelet-derived growth factor
β-thymosin

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title = "Identification of PDGF-BB binding to thymosin β4 by chemical cross-linking",

abstract = "The purpose of our work was to identify unknown interaction partners of thymosin β4 (Tβ4). It was suggested that Tβ4 could be an antifibrotic drug for treatment of liver fibrogenesis, because Tβ4 prevents the platelet-derived growth factor-BB (PDGF-BB)-induced activation of hepatic stellate cells (HSCs). Very little information is available how Tβ4 counteracts the PDGF-BB-induced activation of HSCs. We propose the hypothesis that Tβ4 could bind directly to PDGF-BB and thereby reduce the concentration of free PDGF-BB available for binding to the PDGF-β receptor.",

keywords = "biotin label transfer, cross-linking, platelet-derived growth factor, β-thymosin, biotin label transfer, cross-linking, platelet-derived growth factor, β-thymosin",

author = "Jana Knop and Christine App and Thomas Huff and Federica Iavarone and Massimo Castagnola and Ewald Hannappel",

year = "2015",

doi = "10.1517/14712598.2015.1014793",

language = "English",

volume = "15 Suppl 1",

pages = "S147--S154",

journal = "Expert Opinion on Biological Therapy",

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T1 - Identification of PDGF-BB binding to thymosin β4 by chemical cross-linking

AU - Knop, Jana

AU - App, Christine

AU - Huff, Thomas

AU - Iavarone, Federica

AU - Castagnola, Massimo

AU - Hannappel, Ewald

PY - 2015

Y1 - 2015

N2 - The purpose of our work was to identify unknown interaction partners of thymosin β4 (Tβ4). It was suggested that Tβ4 could be an antifibrotic drug for treatment of liver fibrogenesis, because Tβ4 prevents the platelet-derived growth factor-BB (PDGF-BB)-induced activation of hepatic stellate cells (HSCs). Very little information is available how Tβ4 counteracts the PDGF-BB-induced activation of HSCs. We propose the hypothesis that Tβ4 could bind directly to PDGF-BB and thereby reduce the concentration of free PDGF-BB available for binding to the PDGF-β receptor.

AB - The purpose of our work was to identify unknown interaction partners of thymosin β4 (Tβ4). It was suggested that Tβ4 could be an antifibrotic drug for treatment of liver fibrogenesis, because Tβ4 prevents the platelet-derived growth factor-BB (PDGF-BB)-induced activation of hepatic stellate cells (HSCs). Very little information is available how Tβ4 counteracts the PDGF-BB-induced activation of HSCs. We propose the hypothesis that Tβ4 could bind directly to PDGF-BB and thereby reduce the concentration of free PDGF-BB available for binding to the PDGF-β receptor.

KW - biotin label transfer

KW - cross-linking

KW - platelet-derived growth factor

KW - β-thymosin

KW - biotin label transfer

KW - cross-linking

KW - platelet-derived growth factor

KW - β-thymosin

UR - http://hdl.handle.net/10807/72370

U2 - 10.1517/14712598.2015.1014793

DO - 10.1517/14712598.2015.1014793

M3 - Article

SN - 1471-2598

VL - 15 Suppl 1

SP - S147-S154

JO - Expert Opinion on Biological Therapy

JF - Expert Opinion on Biological Therapy

ER -

Identification of PDGF-BB binding to thymosin β4 by chemical cross-linking

Abstract

Keywords

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