Hybrid artificial pancreas: islet transplantation inside membrane bioreactors

Celestino Pio Lombardi; A Urso; G Careddu; Giovanni Ghirlanda; G Catapano; Giuseppe Brisinda; Franco Ceriati; Rocco Domenico Alfonso Bellantone; Gb Doglietto; F. Crucitti

Hybrid artificial pancreas: islet transplantation inside membrane bioreactors

Celestino Pio Lombardi, A Urso, G Careddu, Giovanni Ghirlanda, G Catapano, Giuseppe Brisinda, Franco Ceriati, Rocco Domenico Alfonso Bellantone, Gb Doglietto, F. Crucitti

Research output: Contribution to journal › Article

Abstract

The use of pancreatic islet transplantation in membrane bioreactors put in vascular circuits aims at resetting the glucose homeostasis in diabetic or pancreatectomized patients, avoiding immune host rejection. Our experience was carried out at following stages: porcine pancreas explantation and enzymatic separation of endocrine tissue from exocrine fraction by collagenase; evaluation of islet functionality (culture tests); in vitro tests of the islets-bioreactor system, to assess the metabolic response to the glucose; in vivo evaluation to assay the haemodynamic behaviour. The trials showed a good metabolic bioreactor functionality and a decreasing incidence of coagulative problems.

Original language	English
Pages (from-to)	1177-1192
Number of pages	16
Journal	Biomaterials, Artificial Cells, and Immobilization Biotechnology
Volume	20
Publication status	Published - 1992

Keywords

Animals
Female
Glucose
Heparin
Insulin
Islets of Langerhans Transplantation
Membranes, Artificial
Swine

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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abstract = "The use of pancreatic islet transplantation in membrane bioreactors put in vascular circuits aims at resetting the glucose homeostasis in diabetic or pancreatectomized patients, avoiding immune host rejection. Our experience was carried out at following stages: porcine pancreas explantation and enzymatic separation of endocrine tissue from exocrine fraction by collagenase; evaluation of islet functionality (culture tests); in vitro tests of the islets-bioreactor system, to assess the metabolic response to the glucose; in vivo evaluation to assay the haemodynamic behaviour. The trials showed a good metabolic bioreactor functionality and a decreasing incidence of coagulative problems.",

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author = "Lombardi, {Celestino Pio} and A Urso and G Careddu and Giovanni Ghirlanda and G Catapano and Giuseppe Brisinda and Franco Ceriati and Bellantone, {Rocco Domenico Alfonso} and Gb Doglietto and F. Crucitti",

year = "1992",

language = "English",

volume = "20",

pages = "1177--1192",

journal = "Biomaterials, Artificial Cells, and Immobilization Biotechnology",

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TY - JOUR

T1 - Hybrid artificial pancreas: islet transplantation inside membrane bioreactors

AU - Lombardi, Celestino Pio

AU - Urso, A

AU - Careddu, G

AU - Ghirlanda, Giovanni

AU - Catapano, G

AU - Brisinda, Giuseppe

AU - Ceriati, Franco

AU - Bellantone, Rocco Domenico Alfonso

AU - Doglietto, Gb

AU - Crucitti, F.

PY - 1992

Y1 - 1992

N2 - The use of pancreatic islet transplantation in membrane bioreactors put in vascular circuits aims at resetting the glucose homeostasis in diabetic or pancreatectomized patients, avoiding immune host rejection. Our experience was carried out at following stages: porcine pancreas explantation and enzymatic separation of endocrine tissue from exocrine fraction by collagenase; evaluation of islet functionality (culture tests); in vitro tests of the islets-bioreactor system, to assess the metabolic response to the glucose; in vivo evaluation to assay the haemodynamic behaviour. The trials showed a good metabolic bioreactor functionality and a decreasing incidence of coagulative problems.

AB - The use of pancreatic islet transplantation in membrane bioreactors put in vascular circuits aims at resetting the glucose homeostasis in diabetic or pancreatectomized patients, avoiding immune host rejection. Our experience was carried out at following stages: porcine pancreas explantation and enzymatic separation of endocrine tissue from exocrine fraction by collagenase; evaluation of islet functionality (culture tests); in vitro tests of the islets-bioreactor system, to assess the metabolic response to the glucose; in vivo evaluation to assay the haemodynamic behaviour. The trials showed a good metabolic bioreactor functionality and a decreasing incidence of coagulative problems.

KW - Animals

KW - Female

KW - Glucose

KW - Heparin

KW - Insulin

KW - Islets of Langerhans Transplantation

KW - Membranes, Artificial

KW - Swine

KW - Animals

KW - Female

KW - Glucose

KW - Heparin

KW - Insulin

KW - Islets of Langerhans Transplantation

KW - Membranes, Artificial

KW - Swine

UR - http://hdl.handle.net/10807/11936

M3 - Article

SN - 1055-7172

VL - 20

SP - 1177

EP - 1192

JO - Biomaterials, Artificial Cells, and Immobilization Biotechnology

JF - Biomaterials, Artificial Cells, and Immobilization Biotechnology

ER -

Hybrid artificial pancreas: islet transplantation inside membrane bioreactors

Abstract

Keywords

UN SDGs

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