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How to assess drug resistance in cancer stem cells

  • Istituto Superiore di Sanita

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.
Original languageEnglish
Title of host publicationCancer Stem Cells: Methods and Protocols, Methods in Molecular Biology,
PublisherHUMANA PRESS INC
Pages107-115
Number of pages9
Volume1692
ISBN (Print)9781493974009
DOIs
Publication statusPublished - 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • CSC
  • Cancer stem cell culture
  • Colorectal cancer
  • Drug resistance
  • Drug screening
  • Genetics
  • In vitro assays
  • Molecular Biology

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