Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.
|Title of host publication||Cancer Stem Cells: Methods and Protocols|
|Number of pages||9|
|Publication status||Published - 2017|
|Name||METHODS IN MOLECULAR BIOLOGY|
- cancer stem cells
- drug screening