High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein.

Giuseppe Maulucci, Marco De Spirito, Giovambattista Pani, Valentina Labate, Marina Mele, Emiliano Panieri, Giuseppe Arcovito, Tommaso Galeotti, Henrik Østergaard, Jakob R. Winther

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

We present the application of a redox-sensitive mutant of the yellow fluorescent protein (rxYFP) to image, with elevated sensitivity and high temporal and spatial resolution, oxidative responses of eukaryotic cells to pathophysiological stimuli. The method presented, based on the ratiometric quantitation of the distribution of fluorescence by confocal microscopy, allows us to draw real-time "redox maps" of adherent cells and to score subtle changes in the intracellular redox state, such as those induced by overexpression of redox-active proteins. This strategy for in vivo imaging of redox signaling circumvents many of the technical limitations currently encountered in the study of complex redox-based phenomena and promises to contribute substantially to this expanding area of signal transduction.
Original languageEnglish
Pages (from-to)75-88
Number of pages14
JournalScience Signaling
Publication statusPublished - 2008

Keywords

  • Confocal microscopy
  • Fluorescent Probe
  • Reactive Oxygen Species
  • Real time Imaging
  • Redox Signaling
  • rxYFP

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