TY - JOUR
T1 - HIGH-DOSE ATORVASTATIN REDUCE OXIDATIVE STRESS OF ISCHEMIA/REPERFUSION INJURY AFTER ISOGENEIC KIDNEY TRANSPLANTATION IN RATS
AU - Cola, E
AU - Cusumano, Giacomo
AU - Romagnoli, Jacopo
AU - Copponi, Giorgia
AU - Citterio, Franco
AU - Caristo, Me
AU - Manchi, Melissa
AU - Severino, Anna
AU - Liuzzo, Giovanna
AU - Crea, Filippo
AU - Capelli, Arnaldo
PY - 2015
Y1 - 2015
N2 - OBJECTIVE:
To investigate the effects of N-acetylcysteine (NAC) and high-dose atorvastatin (ATOR) in reducing oxidative stress in a rat kidney model of ischemia-reperfusion injury.
METHODS:
Forty female rats underwent clamping of the left renal artery for 30 minutes, followed by reperfusion. The effects of pre-ischemic administration of NAC and/or ATOR were evaluated within 4 groups: a) control (no NAC, no ATOR); b) NAC (intraperitoneal NAC administration); c) ATOR (oral ATOR administration); and d) NAC+ATOR (both drugs). Oxidative stress was assessed by measuring the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and myeloperoxidase (MPO). Post-ischemia-reperfusion injury was evaluated by means of renal histology.
RESULTS:
NAC, ATOR, and NAC+ATOR in rats showed lower MPO (P < .05) and higher GPx activity (P < .05) versus control; SOD activity was lower in NAC versus ATOR (P < .05). No difference among groups was found at histology. However, a lower rate of tubular ischemic lesions was evident in NAC+ATOR versus control (P = .07).
CONCLUSIONS:
Atorvastatin pretreatment provides protection against oxidative stress in a rat kidney model of ischemia-reperfusion injury, reinforcing the evidence of a beneficial effect of statins beyond their cholesterol-lowering properties
AB - OBJECTIVE:
To investigate the effects of N-acetylcysteine (NAC) and high-dose atorvastatin (ATOR) in reducing oxidative stress in a rat kidney model of ischemia-reperfusion injury.
METHODS:
Forty female rats underwent clamping of the left renal artery for 30 minutes, followed by reperfusion. The effects of pre-ischemic administration of NAC and/or ATOR were evaluated within 4 groups: a) control (no NAC, no ATOR); b) NAC (intraperitoneal NAC administration); c) ATOR (oral ATOR administration); and d) NAC+ATOR (both drugs). Oxidative stress was assessed by measuring the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and myeloperoxidase (MPO). Post-ischemia-reperfusion injury was evaluated by means of renal histology.
RESULTS:
NAC, ATOR, and NAC+ATOR in rats showed lower MPO (P < .05) and higher GPx activity (P < .05) versus control; SOD activity was lower in NAC versus ATOR (P < .05). No difference among groups was found at histology. However, a lower rate of tubular ischemic lesions was evident in NAC+ATOR versus control (P = .07).
CONCLUSIONS:
Atorvastatin pretreatment provides protection against oxidative stress in a rat kidney model of ischemia-reperfusion injury, reinforcing the evidence of a beneficial effect of statins beyond their cholesterol-lowering properties
KW - ISCHEMIA REPERFUSION
KW - TRANSPLANTATION
KW - ISCHEMIA REPERFUSION
KW - TRANSPLANTATION
UR - http://hdl.handle.net/10807/71796
U2 - 10.1016
DO - 10.1016
M3 - Article
SN - 0041-1345
VL - 2015
SP - 2757
EP - 2762
JO - Transplantation Proceedings
JF - Transplantation Proceedings
ER -