Heterochromatin and rDNA localization on the holocentric chromosomes of black bean aphid, Aphis fabae Scop. (Hemiptera: Aphididae)

Emanuele Mazzoni, Rivil Marco, Stefano Cassanelli, Davide Bizzaro, Gian Carlo Manicardi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Heterochromation and rDNA localization in the holocentric chromosomes of the aphid Aphis fabae has been investigated at a cytological level after C-banding, Giemsa, DAPI NOR staining.C-banding technique showed that heterochromatic areas result mainly clustered on the X chromosomes but, contrary to what observed in other aphid species, in A. fabae C-positive bands are clearly distinguishable also on each autosomal chromosome pair, thus allowing the identification of homologues and a reliable reconstruction of the karyotype. Moreover, silver staining localized rDNA genes on one telomere of each X chromosome; these are the only brightly fluorescent C-positive regions revealed after CMA3 staining, whereas all other heterochromatic bands are DAPI positive. Both CMA3 and silver staining evidenced a noticeable amount of rDNA heteromorphism
Original languageEnglish
Pages (from-to)341-346
Number of pages6
JournalCaryologia
Volume62
Publication statusPublished - 2009

Keywords

  • Aphis fabae
  • afidi
  • aphid
  • citogenetica
  • cromosomi olocentrici
  • cytogenetics
  • holocentric chromosomes

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