Gene expression profiling of myelodysplastic CD34+ hematopoietic stem cells treated in vitro with decitabine

Manuela Giachelia; Francesco D'Alo'; Emiliano Fabiani; Nathalie Saulnier; Annalisa Di Ruscio; Francesco Guidi; Stefan Hohaus; Maria Teresa Voso; Giuseppe Leone

doi:10.1016/j.leukres.2010.07.022

Gene expression profiling of myelodysplastic CD34+ hematopoietic stem cells treated in vitro with decitabine

Manuela Giachelia, Francesco D'Alo', Emiliano Fabiani, Nathalie Saulnier, Annalisa Di Ruscio, Francesco Guidi, Stefan Hohaus, Maria Teresa Voso, Giuseppe Leone

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

Abnormal gene promoter methylation contributes to deregulate gene expression of hematopoietic progenitors in myelodysplastic syndromes (MDS). We analyzed the gene expression profile of myelodysplastic and normal CD34+ hematopoietic stem cells (HSCs) treated in vitro with decitabine. We identified a list of candidate tumor suppressor genes, expressed at low levels in MDS HSCs and induced by hypomethylating treatment only in MDS, but not in normal HSCs. Real-time RT-PCR confirmed reduced CD9 expression in MDS CD34+ and bone marrow mononuclear cells, compared to normal controls. CD9 was specifically up-regulated by decitabine treatment in myelodysplastic CD34+ cells.

Original language	English
Pages (from-to)	465-471
Number of pages	7
Journal	Leukemia Research
Volume	35
DOIs	https://doi.org/10.1016/j.leukres.2010.07.022
Publication status	Published - 2011

Keywords

Adolescent
Adult
Aged
Aged, 80 and over
Antigens, CD29
Antigens, CD34
Antimetabolites, Antineoplastic
Azacitidine
Cluster Analysis
DNA Methylation
Female
Gene Expression
Gene Expression Profiling
Hematopoietic Stem Cells
Humans
Male
Middle Aged
Myelodysplastic Syndromes
Oligonucleotide Array Sequence Analysis
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Young Adult

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10.1016/j.leukres.2010.07.022

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title = "Gene expression profiling of myelodysplastic CD34+ hematopoietic stem cells treated in vitro with decitabine",

abstract = "Abnormal gene promoter methylation contributes to deregulate gene expression of hematopoietic progenitors in myelodysplastic syndromes (MDS). We analyzed the gene expression profile of myelodysplastic and normal CD34+ hematopoietic stem cells (HSCs) treated in vitro with decitabine. We identified a list of candidate tumor suppressor genes, expressed at low levels in MDS HSCs and induced by hypomethylating treatment only in MDS, but not in normal HSCs. Real-time RT-PCR confirmed reduced CD9 expression in MDS CD34+ and bone marrow mononuclear cells, compared to normal controls. CD9 was specifically up-regulated by decitabine treatment in myelodysplastic CD34+ cells.",

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author = "Manuela Giachelia and Francesco D'Alo' and Emiliano Fabiani and Nathalie Saulnier and {Di Ruscio}, Annalisa and Francesco Guidi and Stefan Hohaus and Voso, {Maria Teresa} and Giuseppe Leone",

year = "2011",

doi = "10.1016/j.leukres.2010.07.022",

language = "English",

volume = "35",

pages = "465--471",

journal = "Leukemia Research",

issn = "0145-2126",

publisher = "Oxford,United Kingdom: Elsevier Science Limited -Oxford : Pergamon, 1977-",

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T1 - Gene expression profiling of myelodysplastic CD34+ hematopoietic stem cells treated in vitro with decitabine

AU - Giachelia, Manuela

AU - D'Alo', Francesco

AU - Fabiani, Emiliano

AU - Saulnier, Nathalie

AU - Di Ruscio, Annalisa

AU - Guidi, Francesco

AU - Hohaus, Stefan

AU - Voso, Maria Teresa

AU - Leone, Giuseppe

PY - 2011

Y1 - 2011

N2 - Abnormal gene promoter methylation contributes to deregulate gene expression of hematopoietic progenitors in myelodysplastic syndromes (MDS). We analyzed the gene expression profile of myelodysplastic and normal CD34+ hematopoietic stem cells (HSCs) treated in vitro with decitabine. We identified a list of candidate tumor suppressor genes, expressed at low levels in MDS HSCs and induced by hypomethylating treatment only in MDS, but not in normal HSCs. Real-time RT-PCR confirmed reduced CD9 expression in MDS CD34+ and bone marrow mononuclear cells, compared to normal controls. CD9 was specifically up-regulated by decitabine treatment in myelodysplastic CD34+ cells.

AB - Abnormal gene promoter methylation contributes to deregulate gene expression of hematopoietic progenitors in myelodysplastic syndromes (MDS). We analyzed the gene expression profile of myelodysplastic and normal CD34+ hematopoietic stem cells (HSCs) treated in vitro with decitabine. We identified a list of candidate tumor suppressor genes, expressed at low levels in MDS HSCs and induced by hypomethylating treatment only in MDS, but not in normal HSCs. Real-time RT-PCR confirmed reduced CD9 expression in MDS CD34+ and bone marrow mononuclear cells, compared to normal controls. CD9 was specifically up-regulated by decitabine treatment in myelodysplastic CD34+ cells.

KW - Adolescent

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Antigens, CD29

KW - Antigens, CD34

KW - Antimetabolites, Antineoplastic

KW - Azacitidine

KW - Cluster Analysis

KW - DNA Methylation

KW - Female

KW - Gene Expression

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KW - Antigens, CD34

KW - Antimetabolites, Antineoplastic

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KW - Cluster Analysis

KW - DNA Methylation

KW - Female

KW - Gene Expression

KW - Gene Expression Profiling

KW - Hematopoietic Stem Cells

KW - Humans

KW - Male

KW - Middle Aged

KW - Myelodysplastic Syndromes

KW - Oligonucleotide Array Sequence Analysis

KW - Promoter Regions, Genetic

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Young Adult

UR - http://hdl.handle.net/10807/6620

U2 - 10.1016/j.leukres.2010.07.022

DO - 10.1016/j.leukres.2010.07.022

M3 - Article

SN - 0145-2126

VL - 35

SP - 465

EP - 471

JO - Leukemia Research

JF - Leukemia Research

ER -

Gene expression profiling of myelodysplastic CD34+ hematopoietic stem cells treated in vitro with decitabine

Abstract

Keywords

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