Facts and artifacts in proteomics of body fluids. What proteomics of saliva is telling us?

Irene Messana, Rosanna Inzitari, Chiara Fanali, Tiziana Cabras, Massimo Castagnola

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

This review briefly depicts several salient points of the current status of knowledge on salivary peptidoma. It outlines the intrinsic difficulties in its characterization connected to different factors of variability, such as: i) the high genetic polymorphisms, complicated by individual insertions/deletions and alternative splicing; ii) complex post-translational maturations comprehending different proteolytic cleavages, glycosylation, phosphorylation and sulfation processes; iii) physiological variations and different contributions to the whole. Moreover, several technological and analytical problems and pitfalls that had to be surmounted during our studies focussed on the extensive qualitative and quantitative characterization of salivary peptidoma and mainly based on LC-MS analyses of intact naturally occurring peptides are here described. The hope is that the information provided might be helpful to other groups engaged on the analysis of saliva or other body fluids for clinical applications.
Original languageEnglish
Pages (from-to)1948-1963
Number of pages16
JournalJournal of Separation Science
Publication statusPublished - 2008

Keywords

  • PROTEOMICS
  • SALIVARY

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