Ex vivo expansion of mesenchymal stromal cells

Maria Ester Bernardo; Angela Maria Cometa; Daria Pagliara; Luciana Vinti; Francesca Rossi; Rosaria Cristantielli; Giuseppe Palumbo; Franco Locatelli

doi:10.1016/j.beha.2010.11.002

Ex vivo expansion of mesenchymal stromal cells

Maria Ester Bernardo
, Angela Maria Cometa
, Daria Pagliara
, Luciana Vinti
, Francesca Rossi
, Rosaria Cristantielli
, Giuseppe Palumbo
, Franco Locatelli

IRCCS Ospedale pediatrico Bambino Gesù - Roma
University of Rome Tor Vergata

Research output: Contribution to journal › Article

Abstract

Mesenchymal stromal cells (MSCs) are adult multipotent cells that can be isolated from several human tissues. MSCs represent a novel and attractive tool in strategies of cellular therapy. For in vivo use, MSCs have to be ex vivo expanded in order to reach the numbers suitable for their clinical application. Despite being efficacious, the use of fetal calf serum for MSC ex vivo expansion for clinical purposes raises concerns related to immunization and transmission of zoonoses; the standardization of expansion methods, possibly devoid of animal components, such as those based on platelet lysate, are discussed in this paper. Moreover, this review focuses on the search of novel markers for the prospective identification/isolation of MSCs and on the potential risks connected with ex vivo expansion of MSCs, in particular that of their malignant transformation. Available tests to study the genetic stability of ex vivo expanded MSCs are also analyzed. (C) 2010 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	73-81
Number of pages	9
Journal	BAILLIERE'S BEST PRACTICE IN CLINICAL HAEMATOLOGY
Volume	24
DOIs	https://doi.org/10.1016/j.beha.2010.11.002
Publication status	Published - 2011

Keywords

mesenchymal stromal cells
ex vivo expansion
cellular therapy
platelet lysate
malignant transformation
fetal calf serum

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10.1016/j.beha.2010.11.002

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title = "Ex vivo expansion of mesenchymal stromal cells",

abstract = "Mesenchymal stromal cells (MSCs) are adult multipotent cells that can be isolated from several human tissues. MSCs represent a novel and attractive tool in strategies of cellular therapy. For in vivo use, MSCs have to be ex vivo expanded in order to reach the numbers suitable for their clinical application. Despite being efficacious, the use of fetal calf serum for MSC ex vivo expansion for clinical purposes raises concerns related to immunization and transmission of zoonoses; the standardization of expansion methods, possibly devoid of animal components, such as those based on platelet lysate, are discussed in this paper. Moreover, this review focuses on the search of novel markers for the prospective identification/isolation of MSCs and on the potential risks connected with ex vivo expansion of MSCs, in particular that of their malignant transformation. Available tests to study the genetic stability of ex vivo expanded MSCs are also analyzed. (C) 2010 Elsevier Ltd. All rights reserved.",

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author = "Bernardo, \{Maria Ester\} and Cometa, \{Angela Maria\} and Daria Pagliara and Luciana Vinti and Francesca Rossi and Rosaria Cristantielli and Giuseppe Palumbo and Franco Locatelli",

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doi = "10.1016/j.beha.2010.11.002",

language = "English",

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pages = "73--81",

journal = "BAILLIERE'S BEST PRACTICE IN CLINICAL HAEMATOLOGY",

issn = "1521-6926",

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T1 - Ex vivo expansion of mesenchymal stromal cells

AU - Bernardo, Maria Ester

AU - Cometa, Angela Maria

AU - Pagliara, Daria

AU - Vinti, Luciana

AU - Rossi, Francesca

AU - Cristantielli, Rosaria

AU - Palumbo, Giuseppe

AU - Locatelli, Franco

PY - 2011

Y1 - 2011

N2 - Mesenchymal stromal cells (MSCs) are adult multipotent cells that can be isolated from several human tissues. MSCs represent a novel and attractive tool in strategies of cellular therapy. For in vivo use, MSCs have to be ex vivo expanded in order to reach the numbers suitable for their clinical application. Despite being efficacious, the use of fetal calf serum for MSC ex vivo expansion for clinical purposes raises concerns related to immunization and transmission of zoonoses; the standardization of expansion methods, possibly devoid of animal components, such as those based on platelet lysate, are discussed in this paper. Moreover, this review focuses on the search of novel markers for the prospective identification/isolation of MSCs and on the potential risks connected with ex vivo expansion of MSCs, in particular that of their malignant transformation. Available tests to study the genetic stability of ex vivo expanded MSCs are also analyzed. (C) 2010 Elsevier Ltd. All rights reserved.

AB - Mesenchymal stromal cells (MSCs) are adult multipotent cells that can be isolated from several human tissues. MSCs represent a novel and attractive tool in strategies of cellular therapy. For in vivo use, MSCs have to be ex vivo expanded in order to reach the numbers suitable for their clinical application. Despite being efficacious, the use of fetal calf serum for MSC ex vivo expansion for clinical purposes raises concerns related to immunization and transmission of zoonoses; the standardization of expansion methods, possibly devoid of animal components, such as those based on platelet lysate, are discussed in this paper. Moreover, this review focuses on the search of novel markers for the prospective identification/isolation of MSCs and on the potential risks connected with ex vivo expansion of MSCs, in particular that of their malignant transformation. Available tests to study the genetic stability of ex vivo expanded MSCs are also analyzed. (C) 2010 Elsevier Ltd. All rights reserved.

KW - mesenchymal stromal cells

KW - ex vivo expansion

KW - cellular therapy

KW - platelet lysate

KW - malignant transformation

KW - fetal calf serum

KW - mesenchymal stromal cells

KW - ex vivo expansion

KW - cellular therapy

KW - platelet lysate

KW - malignant transformation

KW - fetal calf serum

UR - http://hdl.handle.net/10807/249174

U2 - 10.1016/j.beha.2010.11.002

DO - 10.1016/j.beha.2010.11.002

M3 - Article

SN - 1521-6926

VL - 24

SP - 73

EP - 81

JO - BAILLIERE'S BEST PRACTICE IN CLINICAL HAEMATOLOGY

JF - BAILLIERE'S BEST PRACTICE IN CLINICAL HAEMATOLOGY

ER -

Ex vivo expansion of mesenchymal stromal cells

Abstract

Keywords

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