Evaluation of the New NucliSENS EasyQ KPC test for rapid detection of Klebsiella pneumoniae carbapenemase genes (blaKPC)

Teresa Spanu, Tiziana D'Inzeo, Ivana Palucci, Mario Tumbarello, Maurizio Sanguinetti, Barbara Fiori Alfarano, Giulia Canu, Serena Campoli, T Giani, Gm Rossolini

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioMérieux EasyQ real-time PCR assay for bla(KPC) detection with 300 members of the Enterobacteriaceae, including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring bla(KPC) genes, with no false positives, and results were available within 2 h.
Original languageEnglish
Pages (from-to)2783-2785
Number of pages3
JournalJournal of Clinical Microbiology
Volume50
DOIs
Publication statusPublished - 2012

Keywords

  • Bacterial Proteins
  • Bacteriological Techniques
  • Enterobacteriaceae
  • Enterobacteriaceae Infections
  • Humans
  • Molecular Diagnostic Techniques
  • Real-Time Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Time Factors
  • beta-Lactam Resistance
  • beta-Lactamases

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