Evaluation of tannin extracts, leonardite and tributyrin supplementation on diarrhoea incidence and gut microbiota of weaned piglets

Maria Luisa Callegari, Matteo Dell’Anno, Serena Reggi, Valentina Caprarulo, Monika Hejna, Carlo Angelo Sgoifo Rossi, Antonella Baldi, Luciana Rossi

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of the dietary administration of a combination of Quebracho and Chestnut tannins, leonardite and tributyrin were evaluated in weaned piglets. A total of 168 weaned piglets (Landrace × Large White) were randomly allotted to two experimental groups (6 pens/group, 14 piglets/pen). Animals were fed a basal control diet (CTRL) and a treatment diet (MIX) supple-mented with 0.75% tannin extracts, 0.25% leonardite and 0.20% tributyrin for 28 days. Individual body weight and feed intake were recorded weekly. Diarrhoea incidence was recorded by a faecal scoring scale (0–3; considering diarrhoea ≥ 2). At 0 and 28 days, faecal samples were obtained from four piglets/pen for microbiological and chemical analyses of faecal microbiota, which were then assessed by V3-V4 region amplification sequencing. At 28 days, blood from two piglets/pen was sampled to evaluate the serum metabolic profile. After 28 days, a reduction in diarrhoea incidence was observed in the MIX compared to CTRL group (p < 0.05). In addition, compared to CTRL, MIX showed a higher lactobacilli:coliform ratio and increased Prevotella and Fibrobacter genera presence (p <0.01). The serum metabolic profile showed a decreased level of low-density lipoproteins in the treated group (p < 0.05). In conclusion, a combination of tannin extract, leonardite and tributyrin could decrease diarrhoea incidence and modulate the gut microbiota.
Original languageEnglish
Pages (from-to)1693-1710
Number of pages18
JournalAnimals
Volume11
DOIs
Publication statusPublished - 2021

Keywords

  • Alternatives to antibiotics
  • Antimicrobial resistance
  • Diarrhoea incidence
  • Feed additives
  • Functional feed
  • Leonardite
  • Microbiota
  • Tannins
  • Tributyrin
  • Weaned piglets

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