Evaluation of multiple stemness markers in normal thyroid and in thyroid carcinoma (TC) specimens

A subset of cells termed cancer stem cells (CSCs) has tumorigenic capacity, also in TC. Iden-tification of a specific antigen to distinguish thyroid CSCs from normal stem cells (SCs) rep-resents a crucial step to develop targeted therapies. In this study, the expression of stemness markers was investigated and putative SCs from both normal thyroid and TC have been iso-lated. Normal and neoplastic tissue samples were harvested from 19 patients who underwent thy-roidectomy. Fresh specimens were enzymatically digested. Cell suspensions were character-ized by flow cytometry using anti-CD133, -CD45, -CD31, -CD44 and -CD105 antibodies. A portion of cells was suspended in SC medium (SCM) in order to obtain thyroid spheres. CD133+ and CD105+ cell rates were significantly higher in TC than in normal thyroid tissue (2.5% vs. 0.1% and 3.9% vs. 0.5% respectively, p<0.02). No difference was found regarding CD44+. In TC both CD45+ and CD31+ cell rates were 1.3%, but 1.45% of CD133+ cells were also positive for CD45 and 0.8% for CD31. In normal tissues, CD45+ and CD31+ cell rates were 0.5% and 0.03%, respectively, but none of CD133+ cells were positive for CD45 and CD31. Isolated putative SCs grew as both free-floating spheres and adherent cells. The re-evaluation of markers in putative SC isolated from 2 cases of normal tissue showed that cells expressed higher levels of CD133, CD105 and CD44 with respect to those obtained after tissue diges-tion. These preliminary findings suggest the presence of a higher fraction of CD133+ and CD105+ cells in TC samples compared to normal tissues. The higher expression of the stemness markers in TC may be related to the better survival of cells obtained from the TC samples in the SCM. Further investigation is required to draw any conclusion, but our approach seems to be promising. Funded by a D.1 grant 2013