Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study

Mozhgan Boroumand; Vincenzo M. Grassi; Flaminia Castagnola; Fabio De Giorgio; Ernesto D'Aloja; Giuseppe Vetrugno; Vincenzo Lorenzo Pascali; Federica Vincenzoni; Federica Iavarone; Gavino Faa; Massimo Castagnola

doi:10.1016/j.ijms.2022.116952

Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study

Mozhgan Boroumand, Vincenzo M. Grassi, Flaminia Castagnola, Fabio De Giorgio, Ernesto D'Aloja, Giuseppe Vetrugno, Vincenzo Lorenzo Pascali, Federica Vincenzoni, Federica Iavarone, Gavino Faa, Massimo Castagnola

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Abstract

This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	N/A-N/A
Journal	International Journal of Mass Spectrometry
Volume	483
DOIs	https://doi.org/10.1016/j.ijms.2022.116952
Publication status	Published - 2023

Keywords

Forensic autopsy
Peptide fragments
Postmortem interval
Postmortem investigation
Time of death
Top-down HPLC-MS analysis
Vitreous humour

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10.1016/j.ijms.2022.116952

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Boroumand, M., Grassi, V. M., Castagnola, F., De Giorgio, F., D'Aloja, E., Vetrugno, G., Pascali, V. L., Vincenzoni, F., Iavarone, F., Faa, G., & Castagnola, M. (2023). Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study. International Journal of Mass Spectrometry, 483, N/A-N/A. https://doi.org/10.1016/j.ijms.2022.116952

@article{49d5e79c311245cfad94cb6fa13c5d98,

title = "Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study",

abstract = "This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.",

keywords = "Forensic autopsy, Peptide fragments, Postmortem interval, Postmortem investigation, Time of death, Top-down HPLC-MS analysis, Vitreous humour, Forensic autopsy, Peptide fragments, Postmortem interval, Postmortem investigation, Time of death, Top-down HPLC-MS analysis, Vitreous humour",

author = "Mozhgan Boroumand and Grassi, {Vincenzo M.} and Flaminia Castagnola and {De Giorgio}, Fabio and Ernesto D'Aloja and Giuseppe Vetrugno and Pascali, {Vincenzo Lorenzo} and Federica Vincenzoni and Federica Iavarone and Gavino Faa and Massimo Castagnola",

year = "2023",

doi = "10.1016/j.ijms.2022.116952",

language = "English",

volume = "483",

pages = "N/A--N/A",

journal = "International Journal of Mass Spectrometry",

issn = "1387-3806",

publisher = "Amsterdam ; Lausanne ; New York ; Oxford ; Shannon ; Tokyo : Elsevier",

}

Boroumand, M, Grassi, VM, Castagnola, F, De Giorgio, F, D'Aloja, E, Vetrugno, G , Pascali, VL, Vincenzoni, F, Iavarone, F, Faa, G & Castagnola, M 2023, 'Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study', International Journal of Mass Spectrometry, vol. 483, pp. N/A-N/A. https://doi.org/10.1016/j.ijms.2022.116952

TY - JOUR

T1 - Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study

AU - Boroumand, Mozhgan

AU - Grassi, Vincenzo M.

AU - Castagnola, Flaminia

AU - De Giorgio, Fabio

AU - D'Aloja, Ernesto

AU - Vetrugno, Giuseppe

AU - Pascali, Vincenzo Lorenzo

AU - Vincenzoni, Federica

AU - Iavarone, Federica

AU - Faa, Gavino

AU - Castagnola, Massimo

PY - 2023

Y1 - 2023

N2 - This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.

AB - This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.

KW - Forensic autopsy

KW - Peptide fragments

KW - Postmortem interval

KW - Postmortem investigation

KW - Time of death

KW - Top-down HPLC-MS analysis

KW - Vitreous humour

KW - Forensic autopsy

KW - Peptide fragments

KW - Postmortem interval

KW - Postmortem investigation

KW - Time of death

KW - Top-down HPLC-MS analysis

KW - Vitreous humour

UR - http://hdl.handle.net/10807/262300

U2 - 10.1016/j.ijms.2022.116952

DO - 10.1016/j.ijms.2022.116952

M3 - Article

SN - 1387-3806

VL - 483

SP - N/A-N/A

JO - International Journal of Mass Spectrometry

JF - International Journal of Mass Spectrometry

ER -

Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study

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Keywords

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