Abstract
Permanent cell cultures are invaluable tools for understanding the biological characteristics of tumors. In the present study the authors report on the establishment of permanent human cell lines from three cases of aggressive chordomas of the clival region. All of the parental tumors showed telomerase activity. Cultured chordoma cells had a doubling time of 5 to 7 days and grew as a monolayer of cells that retained both the immunophenotype and the p53 status of the parental tumor. In vitro, chordoma cells overexpressed telomerase, supporting the hypothesis that this enzyme is required for the immortalization process.
| Original language | English |
|---|---|
| Pages (from-to) | 482-484 |
| Number of pages | 3 |
| Journal | Journal of Neurosurgery |
| Volume | 105 |
| DOIs | |
| Publication status | Published - 2006 |
Keywords
- Adult
- Cell Line, Tumor
- Cell culture
- Chordoma
- Cranial Fossa, Posterior
- Female
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Neurology (clinical)
- Neuroscience (all)
- Skull Base Neoplasms
- Skull base tumor
- Telomerase