Epigenetic Barcodes for Detection of Adulterated Plants and Plant-Derived Products

Matteo Busconi; Giovanna Soffritti; Marcelino De Los Mozos Pascual; José Antonio Fernandez

doi:10.1007/978-1-0716-0179-2_16

Epigenetic Barcodes for Detection of Adulterated Plants and Plant-Derived Products

Matteo Busconi^*, Giovanna Soffritti, Marcelino De Los Mozos Pascual, José Antonio Fernandez

^*Corresponding author

Research output: Contribution to journal › Article

Abstract

In this chapter, we report a possible alternative use of epigenetics by applying methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) to saffron traceability. Saffron is the most expensive plant-derived product in the world and one of the most frequently adulterated. One of the most frequent adulteration is by adding to saffron stigmas different parts of the saffron flower itself to increase volumes. While DNA is the same in all the parts of the plant, the epigenetic state can vary according to the organ and/or tissue of origin, making it possible to discriminate the stigmas from the other parts of saffron flower. In the subsequent method, the protocol to carry out a MS-AFLP analysis of saffron DNA methylation patterns is described.

Original language	English
Pages (from-to)	227-242
Number of pages	16
Journal	Methods in Molecular Biology
Volume	2093
DOIs	https://doi.org/10.1007/978-1-0716-0179-2_16
Publication status	Published - 2020

Keywords

Epigenetics
MS-AFLP
Saffron
Traceability

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10.1007/978-1-0716-0179-2_16

Cite this

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title = "Epigenetic Barcodes for Detection of Adulterated Plants and Plant-Derived Products",

abstract = "In this chapter, we report a possible alternative use of epigenetics by applying methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) to saffron traceability. Saffron is the most expensive plant-derived product in the world and one of the most frequently adulterated. One of the most frequent adulteration is by adding to saffron stigmas different parts of the saffron flower itself to increase volumes. While DNA is the same in all the parts of the plant, the epigenetic state can vary according to the organ and/or tissue of origin, making it possible to discriminate the stigmas from the other parts of saffron flower. In the subsequent method, the protocol to carry out a MS-AFLP analysis of saffron DNA methylation patterns is described.",

keywords = "Epigenetics, MS-AFLP, Saffron, Traceability, Epigenetics, MS-AFLP, Saffron, Traceability",

author = "Matteo Busconi and Giovanna Soffritti and {Mozos Pascual}, {Marcelino De Los} and Fernandez, {Jos{\'e} Antonio}",

year = "2020",

doi = "10.1007/978-1-0716-0179-2_16",

language = "English",

volume = "2093",

pages = "227--242",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

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TY - JOUR

T1 - Epigenetic Barcodes for Detection of Adulterated Plants and Plant-Derived Products

AU - Busconi, Matteo

AU - Soffritti, Giovanna

AU - Mozos Pascual, Marcelino De Los

AU - Fernandez, José Antonio

PY - 2020

Y1 - 2020

N2 - In this chapter, we report a possible alternative use of epigenetics by applying methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) to saffron traceability. Saffron is the most expensive plant-derived product in the world and one of the most frequently adulterated. One of the most frequent adulteration is by adding to saffron stigmas different parts of the saffron flower itself to increase volumes. While DNA is the same in all the parts of the plant, the epigenetic state can vary according to the organ and/or tissue of origin, making it possible to discriminate the stigmas from the other parts of saffron flower. In the subsequent method, the protocol to carry out a MS-AFLP analysis of saffron DNA methylation patterns is described.

AB - In this chapter, we report a possible alternative use of epigenetics by applying methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) to saffron traceability. Saffron is the most expensive plant-derived product in the world and one of the most frequently adulterated. One of the most frequent adulteration is by adding to saffron stigmas different parts of the saffron flower itself to increase volumes. While DNA is the same in all the parts of the plant, the epigenetic state can vary according to the organ and/or tissue of origin, making it possible to discriminate the stigmas from the other parts of saffron flower. In the subsequent method, the protocol to carry out a MS-AFLP analysis of saffron DNA methylation patterns is described.

KW - Epigenetics

KW - MS-AFLP

KW - Saffron

KW - Traceability

KW - Epigenetics

KW - MS-AFLP

KW - Saffron

KW - Traceability

UR - http://hdl.handle.net/10807/151593

U2 - 10.1007/978-1-0716-0179-2_16

DO - 10.1007/978-1-0716-0179-2_16

M3 - Article

SN - 1064-3745

VL - 2093

SP - 227

EP - 242

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Epigenetic Barcodes for Detection of Adulterated Plants and Plant-Derived Products

Abstract

Keywords

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