Effect of the presence of two commercial adsorbents in animal feed on aflatoxin B1 detrmination by ELISA kit test.

A rapid AFB1 detection method by ELISA kit test was used on feedstuff samples, and\r\ncompared to an HPLC method, to verify if the presence of clay-adsorbent (SA) could cause erroneous\r\nquantification of the toxin. Samples were obtained using two AFB1-contaminated feedstuffs (7.92 and\r\n17.58 µg/kg for low and high contaminated feeds; LC and HC respectively), added either one of two\r\ncommercial SAs (Atox® and Myco AD) and three different inclusion doses (0, 10 and 20 g/kg, respectively\r\nfor CTR, 1% and 2% doses). The HPLC and ELISA data were compared in CTR samples with a\r\npaired t-test. The AFB1 recoveries, performed with ELISA, were analysed as a completely randomized\r\ndesign using a 2×2×3 factorial arrangement. The ELISA method tended to underestimate the AFB1\r\nconcentrations with respect to the HPLC method, both in HC (P=0.050) and in LC (P<0.001) feedstuffs.\r\nA more drastic reduction (P<0.001) was observed when SAs were included in the two feedstuffs.\r\nIn particular, Atox® determined an AFB1 recovery of 15,5% in HC and 7,6% in LC (1% dose) and of\r\n11,1% in HC and 8,4% in LC (2% dose).