Effect of pro-inflammatory/anti-inflammatory agents on cytokine secretion by peripheral blood mononuclear cells in rheumatoid arthritis and systemic lupus erythematosus

Carlo Provenzano, Mariapaola Marino, Angelo Zoli, Flavia Scuderi, Emanuela Bartoccioni

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We studied a well-selected population of patients with active rheumatoid arthritis (RA) or systemic lupus erythematosus (SLE) without immunosuppressive therapy. Control and patient peripheral blood mononuclear cells (PBMC) were incubated with IL-1β, IL-10, TGF-β or LPS for 20 h and the in vitro basal and stimulated secretions of IL-6, TNF-α, IL-1β and IL-1ra were measured by ELISA. We found that in the SLE patients the basal secretion of IL-6 was significantly lower and that of IL-1ra significantly higher than in control subjects, while in the RA group the basal IL-1ra secretion was higher than in healthy subjects. SLE and RA PBMC responded to LPS and IL-1β reaching higher cytokine secretion values man controls. The in vitro response of SLE and RA PBMC to TGFβ was normal, while that to IL-10 was defective: IL-10 was able to stimulate the production of IL-6 and IL-1ra in PBMC from normal subjects, but it was unable to enhance IL-6 secretion in RA cells and it was also completely ineffective in inducing IL-1ra secretion in both SLE and RA PBMC. Our work add new data useful for the evaluation of IL-10 and IL-1ra as therapeutic agents in rheumatic diseases.
Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalAutoimmunity
Volume36
DOIs
Publication statusPublished - 2003

Keywords

  • Adolescent
  • Adult
  • Anti-Inflammatory Agents, Non-Steroidal
  • Arthritis, Rheumatoid
  • Case-Control Studies
  • Cytokines
  • Female
  • Humans
  • IL-1
  • IL-10
  • In Vitro Techniques
  • Inflammation Mediators
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Interleukin-10
  • Interleukin-6
  • Leukocytes, Mononuclear
  • Lipopolysaccharides
  • Lupus Erythematosus, Systemic
  • Middle Aged
  • Mononuclear cells
  • Recombinant Proteins
  • Rheumatoid arthritis
  • SLE
  • Sialoglycoproteins
  • Transforming Growth Factor beta
  • Transforming growth factors
  • Tumor Necrosis Factor-alpha

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