EFFECT OF 2-METHOXY-5-NITROTROPONE ON OXYGEN-AFFINITY OF HUMAN ERYTHROCYTES AND HEMOGLOBIN

D Currell, E Benitez, C Ioppolo, Bruno Giardina, S Condo, F Martini, E. Antonini

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Human hemoglobin reacted with 2-methoxy-5-nitrotropone at pH 7.4 undergoes modification of the four N-terminal amino groups. The modified protein shows increased oxygen affinity with complete abolition of the effect of D-glycerate 2,3-bisphosphate. The Bohr effect is abolished in the acid range and drastically reduced at alkaline pH values. Changes in the Kinetics of ligand reactions parallel the oxygen equilibrium results. Cooperative effects are still present. Human erythrocytes treated with 2-methoxy-5-nitrotropone show increased oxygen affinity and some decrease in methemoglobin reductase efficiency but no change in resistance to hemolysis
Original languageEnglish
Pages (from-to)285-289
Number of pages5
JournalEuropean Journal of Biochemistry
Volume91
DOIs
Publication statusPublished - 1978
Externally publishedYes

Keywords

  • HEMOGLOBIN
  • HUMAN HERYTHROCYTES

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