Dynamic light scattering for the characterization and counting of extracellular vesicles: a powerful noninvasive tool

Donatella Lucchetti, Giuseppe Maulucci, Massimiliano Papi, Roberto Pola, Marco De Spirito, Alessandro Sgambato, Valentina Palmieri, Ilaria Gatto, Alessandro Maiorana, Margherita Marcantoni

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Extracellular vesicles (EVs) are cell-to-cell shuttles that have recently drawn interest both as drug delivery platforms and disease biomarkers. Despite the increasingly recognized relevance of these vesicles, their detection, and characterization still have several technical drawbacks. In this paper, we accurately assess the size distribution and concentration of EVs by using a high-throughput non-perturbative technique such as Dynamic Light Scattering (DLS). The vesicle radii distribution, as further confirmed by Atomic Force Microscopy experiments, ranges from 10 to 80 nm and appears very asymmetric towards larger radii with a main peak at roughly 30 nm. By combining DLS and Bradford assay, we also demonstrate the feasibility of recovering the concentration and its distribution of proteins contained inside vesicles. The sensitivity of our approach allows to detect protein concentrations as low as 0.01 mg/ml.
Original languageEnglish
Pages (from-to)N/A-N/A
JournalJournal of Nanoparticle Research
Volume16
DOIs
Publication statusPublished - 2014

Keywords

  • dynamic light scattering

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