Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Giulia De Angelis; Riccardo Paggi; Raffaela Paggi; Thomas J. Lowery; Jessica L. Snyder; Giulia Menchinelli; Maurizio Sanguinetti; Brunella Posteraro; Antonella Mencacci

doi:10.3390/antibiotics10080950

Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Giulia De Angelis, Riccardo Paggi, Raffaela Paggi, Thomas J. Lowery, Jessica L. Snyder, Giulia Menchinelli, Maurizio Sanguinetti, Brunella Posteraro, Antonella Mencacci

University of Perugia

Research output: Contribution to journal › Article

Abstract

Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

Original language	English
Pages (from-to)	950-N/A
Journal	Antibiotics
Volume	10
DOIs	https://doi.org/10.3390/antibiotics10080950
Publication status	Published - 2021

Keywords

Antimicrobial resistance
Blood sample
Bloodstream infection
Direct detection
KPC carbapenemase
Magnetic resonance
T2Resistance panel

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10.3390/antibiotics10080950

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title = "Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay",

abstract = "Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance{\texttrademark} panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance{\texttrademark} panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.",

keywords = "Antimicrobial resistance, Blood sample, Bloodstream infection, Direct detection, KPC carbapenemase, Magnetic resonance, T2Resistance panel, Antimicrobial resistance, Blood sample, Bloodstream infection, Direct detection, KPC carbapenemase, Magnetic resonance, T2Resistance panel",

author = "{De Angelis}, Giulia and Riccardo Paggi and Raffaela Paggi and Lowery, {Thomas J.} and Snyder, {Jessica L.} and Giulia Menchinelli and Maurizio Sanguinetti and Brunella Posteraro and Antonella Mencacci",

year = "2021",

doi = "10.3390/antibiotics10080950",

language = "English",

volume = "10",

pages = "950--N/A",

journal = "Antibiotics",

issn = "2079-6382",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

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TY - JOUR

T1 - Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

AU - De Angelis, Giulia

AU - Paggi, Riccardo

AU - Paggi, Raffaela

AU - Lowery, Thomas J.

AU - Snyder, Jessica L.

AU - Menchinelli, Giulia

AU - Sanguinetti, Maurizio

AU - Posteraro, Brunella

AU - Mencacci, Antonella

PY - 2021

Y1 - 2021

N2 - Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

AB - Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

KW - Antimicrobial resistance

KW - Blood sample

KW - Bloodstream infection

KW - Direct detection

KW - KPC carbapenemase

KW - Magnetic resonance

KW - T2Resistance panel

KW - Antimicrobial resistance

KW - Blood sample

KW - Bloodstream infection

KW - Direct detection

KW - KPC carbapenemase

KW - Magnetic resonance

KW - T2Resistance panel

UR - http://hdl.handle.net/10807/198167

U2 - 10.3390/antibiotics10080950

DO - 10.3390/antibiotics10080950

M3 - Article

SN - 2079-6382

VL - 10

SP - 950-N/A

JO - Antibiotics

JF - Antibiotics

ER -

Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Abstract

Keywords

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