Direct determination of small RNAs using a biotinylated polythiophene impedimetric genosensor

Diego Voccia, Marta Sosnowska, Francesca Bettazzi, Giuseppina Roscigno, Emiliano Fratini, Vittorio De Franciscis, Gerolama Condorelli, Raghu Chitta, Francis D'Souza, Wlodzimierz Kutner, Ilaria Palchetti

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Herein, direct determination of small RNAs is described using a functional-polymer modified genosensor. The analytical strategy adopted involves deposition by electropolymerization of biotinylated polythiophene films on the surface of miniaturized, disposable, gold screen-printed electrodes, followed by the layer-by-layer deposition of streptavidin, and then biotynilated capture probes. A small RNA (miR-221) target was determined via the impedimetric measurement of the hybridization event in a label-free and PCR-free approach. Under optimized conditions, the limit of detection (LOD) was 0.7 pM miR-221 (15% RSD). The genosensor was applied for determination of miR-221 in total RNA extracted from human lung and breast cancer cell lines, discriminating between the cancer-positive and -negative cells, without any amplification step, in less than 2 h.
Original languageEnglish
Pages (from-to)1012-1019
Number of pages8
JournalBIOSENSORS & BIOELECTRONICS
Volume87
DOIs
Publication statusPublished - 2017

Keywords

  • 2′-bithien-5-yl)methane
  • Biosensing Techniques
  • Biotinylation
  • Bis(2
  • Cell Line, Tumor
  • Dielectric Spectroscopy
  • Electrochemical impedance spectroscopy
  • Genosensor
  • Humans
  • Limit of Detection
  • MicroRNAs
  • Neoplasms
  • Polymers
  • Small RNA
  • Thiophenes
  • microRNA

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