Different detection capabilities by mycological media for Candida isolates from mono- or dual-species cultures

Giulia De Angelis; Giulia Menchinelli; Riccardo Torelli; Elena De Carolis; Patrizia Posteraro; Maurizio Sanguinetti; Brunella Posteraro

doi:10.1371/journal.pone.0226467

Different detection capabilities by mycological media for Candida isolates from mono- or dual-species cultures

Giulia De Angelis, Giulia Menchinelli, Riccardo Torelli, Elena De Carolis, Patrizia Posteraro, Maurizio Sanguinetti, Brunella Posteraro

GVM Ospedale San Carlo di Nancy

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Abstract

The aim of this study was to compare the Candida bromcresol green (BCG) medium with the chromogenic (CHROM) Brilliance Candida agar and Sabouraud dextrose agar (SDA) media in regard to their capability of detecting Candida isolates from mono- or dual-species cultures. We prepared Candida isolates' suspensions to obtain mono-species (n = 18) or dual-species (n = 153) culture plates per each medium, and three readers independently observed 513 plates at 24-h, 48-h and 72-h incubation time. We scored reading results as correct, over or under detection compared to the expected species number(s). BCG showed significantly higher correct-detection and lower under-detection rates for all Candida species when observed by at least one reader. At 24-h reading, 12 mono-species cultures had correct (or over) detections in all media, whereas 106, 60 and 78 dual-species cultures had correct (or over) detections in BCG, CHROM or SDA, respectively. BCG provides the basis for an accurate laboratory diagnosis of Candida infections.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	PLoS One
Volume	15
DOIs	https://doi.org/10.1371/journal.pone.0226467
Publication status	Published - 2020

Keywords

Culture media

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title = "Different detection capabilities by mycological media for Candida isolates from mono- or dual-species cultures",

abstract = "The aim of this study was to compare the Candida bromcresol green (BCG) medium with the chromogenic (CHROM) Brilliance Candida agar and Sabouraud dextrose agar (SDA) media in regard to their capability of detecting Candida isolates from mono- or dual-species cultures. We prepared Candida isolates' suspensions to obtain mono-species (n = 18) or dual-species (n = 153) culture plates per each medium, and three readers independently observed 513 plates at 24-h, 48-h and 72-h incubation time. We scored reading results as correct, over or under detection compared to the expected species number(s). BCG showed significantly higher correct-detection and lower under-detection rates for all Candida species when observed by at least one reader. At 24-h reading, 12 mono-species cultures had correct (or over) detections in all media, whereas 106, 60 and 78 dual-species cultures had correct (or over) detections in BCG, CHROM or SDA, respectively. BCG provides the basis for an accurate laboratory diagnosis of Candida infections.",

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author = "{De Angelis}, Giulia and Giulia Menchinelli and Riccardo Torelli and {De Carolis}, Elena and Patrizia Posteraro and Maurizio Sanguinetti and Brunella Posteraro",

year = "2020",

doi = "10.1371/journal.pone.0226467",

language = "English",

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T1 - Different detection capabilities by mycological media for Candida isolates from mono- or dual-species cultures

AU - De Angelis, Giulia

AU - Menchinelli, Giulia

AU - Torelli, Riccardo

AU - De Carolis, Elena

AU - Posteraro, Patrizia

AU - Sanguinetti, Maurizio

AU - Posteraro, Brunella

PY - 2020

Y1 - 2020

N2 - The aim of this study was to compare the Candida bromcresol green (BCG) medium with the chromogenic (CHROM) Brilliance Candida agar and Sabouraud dextrose agar (SDA) media in regard to their capability of detecting Candida isolates from mono- or dual-species cultures. We prepared Candida isolates' suspensions to obtain mono-species (n = 18) or dual-species (n = 153) culture plates per each medium, and three readers independently observed 513 plates at 24-h, 48-h and 72-h incubation time. We scored reading results as correct, over or under detection compared to the expected species number(s). BCG showed significantly higher correct-detection and lower under-detection rates for all Candida species when observed by at least one reader. At 24-h reading, 12 mono-species cultures had correct (or over) detections in all media, whereas 106, 60 and 78 dual-species cultures had correct (or over) detections in BCG, CHROM or SDA, respectively. BCG provides the basis for an accurate laboratory diagnosis of Candida infections.

AB - The aim of this study was to compare the Candida bromcresol green (BCG) medium with the chromogenic (CHROM) Brilliance Candida agar and Sabouraud dextrose agar (SDA) media in regard to their capability of detecting Candida isolates from mono- or dual-species cultures. We prepared Candida isolates' suspensions to obtain mono-species (n = 18) or dual-species (n = 153) culture plates per each medium, and three readers independently observed 513 plates at 24-h, 48-h and 72-h incubation time. We scored reading results as correct, over or under detection compared to the expected species number(s). BCG showed significantly higher correct-detection and lower under-detection rates for all Candida species when observed by at least one reader. At 24-h reading, 12 mono-species cultures had correct (or over) detections in all media, whereas 106, 60 and 78 dual-species cultures had correct (or over) detections in BCG, CHROM or SDA, respectively. BCG provides the basis for an accurate laboratory diagnosis of Candida infections.

KW - Culture media

UR - http://hdl.handle.net/10807/166924

U2 - 10.1371/journal.pone.0226467

DO - 10.1371/journal.pone.0226467

M3 - Article

SN - 1932-6203

VL - 15

SP - 1

EP - 9

JO - PLoS One

JF - PLoS One

ER -

Different detection capabilities by mycological media for Candida isolates from mono- or dual-species cultures

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Keywords

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