Different detection capabilities by mycological media for Candida isolates from mono- or dual-species cultures

Giulia De Angelis, Maurizio Sanguinetti, Brunella Posteraro, Giulia Menchinelli, Riccardo Torelli, Elena De Carolis, Patrizia Posteraro

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The aim of this study was to compare the Candida bromcresol green (BCG) medium with the chromogenic (CHROM) Brilliance Candida agar and Sabouraud dextrose agar (SDA) media in regard to their capability of detecting Candida isolates from mono- or dual-species cultures. We prepared Candida isolates' suspensions to obtain mono-species (n = 18) or dual-species (n = 153) culture plates per each medium, and three readers independently observed 513 plates at 24-h, 48-h and 72-h incubation time. We scored reading results as correct, over or under detection compared to the expected species number(s). BCG showed significantly higher correct-detection and lower under-detection rates for all Candida species when observed by at least one reader. At 24-h reading, 12 mono-species cultures had correct (or over) detections in all media, whereas 106, 60 and 78 dual-species cultures had correct (or over) detections in BCG, CHROM or SDA, respectively. BCG provides the basis for an accurate laboratory diagnosis of Candida infections.
Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalPLoS One
Volume15
DOIs
Publication statusPublished - 2020

Keywords

  • Culture media

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