TY - JOUR
T1 - Determination of polyphenols using liquid chromatography–tandem mass spectrometry technique (LC–MS/MS): A review
AU - López-Fernández, Olalla
AU - Domínguez, Rubén
AU - Pateiro, Mirian
AU - Munekata, Paulo E. S.
AU - Rocchetti, Gabriele
AU - Lorenzo, José M.
PY - 2020
Y1 - 2020
N2 - In recent years, the consumption of polyphenols has been increasing, largely due to its beneficial effects on health. They are present in a wide variety of foods, but their extraction and characterization are complicated since they are mostly in complex matrices. For this reason, the use of selective, sensitive, and versatile analytical techniques such as liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) is necessary. In this review, the most relevant studies of the last years regarding the analysis of polyphenols in different matrices by comprehensive LC– MS/MS are discussed. Relevant steps such as extraction, sample purification, and chromatographic analysis methods are emphasized. In particular, the following methodological aspects are discussed: (a) the proper selection of the extraction technique, (b) the extraction and elution solvents, (c) the purification step, (d) the selection of both stationary and mobile phases for the chromatographic separation of compounds, and (e) the different conditions for mass spectrometry. Overall, this review presents the data from the most recent studies, in a comprehensive way, thus providing and simplifying the information of the great variety of works that exist in the literature on this wide topic.
AB - In recent years, the consumption of polyphenols has been increasing, largely due to its beneficial effects on health. They are present in a wide variety of foods, but their extraction and characterization are complicated since they are mostly in complex matrices. For this reason, the use of selective, sensitive, and versatile analytical techniques such as liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) is necessary. In this review, the most relevant studies of the last years regarding the analysis of polyphenols in different matrices by comprehensive LC– MS/MS are discussed. Relevant steps such as extraction, sample purification, and chromatographic analysis methods are emphasized. In particular, the following methodological aspects are discussed: (a) the proper selection of the extraction technique, (b) the extraction and elution solvents, (c) the purification step, (d) the selection of both stationary and mobile phases for the chromatographic separation of compounds, and (e) the different conditions for mass spectrometry. Overall, this review presents the data from the most recent studies, in a comprehensive way, thus providing and simplifying the information of the great variety of works that exist in the literature on this wide topic.
KW - Analytical methods
KW - Anthocyanins
KW - Phenolic compounds
KW - Flavonols
KW - LC-MS/MS
KW - Electrospray ionization
KW - Analytical methods
KW - Anthocyanins
KW - Phenolic compounds
KW - Flavonols
KW - LC-MS/MS
KW - Electrospray ionization
UR - http://hdl.handle.net/10807/258065
U2 - 10.3390/antiox9060479
DO - 10.3390/antiox9060479
M3 - Article
SN - 2076-3921
VL - 9
SP - N/A-N/A
JO - Antioxidants
JF - Antioxidants
ER -