Cytogenetic investigations on the holocentric chromosomes of Myzus varians and Myzus cerasi have been carried out using silver staining and C-banding followed by chromomycin A3 (CMA3) and DAPI staining in order to improve our knowledge of these pest crop species and facilitate their identification. In M. varians C-banding pattern is pecu-: liar of each chromosome pair thus allowing the identification of all homologues and the reconstruction of a reliable karyotype whereas in M. cerasi, C-positive regions result to be limited to both the telomeric regions of the X chromosomes. In both species, silver staining localizes rDNA genes on one telomere of each X chromosome; such telomeres are the unique brightly fluorescent C-positive regions after CMA3 staining, whereas all other heterochromatic bands result DAPI positive.
|Number of pages||5|
|Publication status||Published - 1999|
- Holocentric chromosomes