Confronto dell’antibioticoresistenza di ceppi di Pseudomonas aeruginosa e di batteri Gram negativi non fermentanti: uno studio cross sectional condotto su isolati da matrici ambientali in un Policlinico Universitario di Roma

Translated title of the contribution: [Autom. eng. transl.] Comparison of the antibiotic resistance of Pseudomonas aeruginosa strains and non-fermenting Gram negative bacteria: a cross sectional study conducted on isolates from environmental matrices in a University Hospital of Rome

Stefania Bruno, Gianluigi Quaranta, Patrizia Laurenti, Gualtiero Ricciardi, Serena Carovillano, Francesco Dalla Torre Del Tempio Di Sanguinetto, Cinzia Turnaturi, Maria Giovanna Ficarra

Research output: Contribution to journalConference article

Abstract

[Autom. eng. transl.] SPECIFIC OBJECTIVES: Recent studies have focused attention on the role of water, in favoring, through the exchange of genes, the onset and spread of antibiotic resistance. In order to evaluate the antibiotic resistance of Pseudomonas aeruginosa, a cross-sectional study of the resistance of strains of this microorganism was compared with non-fermenting Gram-negative bacteria (BGNnF) isolated in samples of “aqueous” environmental matrices, in the September period 2004-May 2010, assuming environmental anti-antibiotic resistance as a proxy for clinical resistance. MATERIALS AND METHODS: In medium and high-risk departments of a University Hospital in Rome, samples of aqueous matrices were taken for BGNnF research by direct filtration or seeding on selective medium and the susceptibility of the isolates to 19 antibiotics individually and tested. multiresistance to two, three and four antibiotics. The strains were subjected to resistance studies with miniaturized automatic systems (ATB PSE 5, BioMèrieux). The chi-square test was used to evaluate the association between the multi-antibiotic resistance of P. aeruginosa and that of BGNnF; the statistical significance was set at a value of p <0.05. RESULTS: 2,424 samples were taken: 648 of tap water, 1,314 of water for the preparation of dialysate and 462 of liquid for the verification of the effectiveness of disinfection of bronchoscopes. Of the 115 isolated strains 39 (33.91%) were P. aeruginosa and 76 (66.08%) BGNnF. The results showing a significant association of P. aeruginosa resistance with BGNnF are reported: in relation to P. aeruginosa 15.8% (n = 6) of the strains was resistant to Amikacin (p <0.001), 14.3% (n = 11) to Cotrimoxazole (p <0.001), 15.8% (n = 6) to Gentamicin (p <0.001), 15.8% (n = 6) to Tobramycin (p <0.001), 48.7% (n = 38) to Ampicillin-Sulbactam (p <0.001). Furthermore, 36.9% (n = 38) of P. aeruginosa strains was resistant to two antibiotics (p <0.001), and 42.1% (n = 16) to four antibiotics (p = 0.02 ). CONCLUSIONS: Although the association of resistance to the latest antibiotics does not show significant values compared to other BGNnF, the significance of the association of P. aeruginosa multiresistances must be carefully evaluated. This requires a more in-depth reflection on the phenomenon of resistance in environmental matrices, such as proxies of clinical resistance, the comparison of which becomes necessary, together with a monitoring of resistance and a rational use of antibiotics.
Translated title of the contribution[Autom. eng. transl.] Comparison of the antibiotic resistance of Pseudomonas aeruginosa strains and non-fermenting Gram negative bacteria: a cross sectional study conducted on isolates from environmental matrices in a University Hospital of Rome
Original languageItalian
Pages (from-to)510-510
Number of pages1
JournalIGIENE E SANITÀ PUBBLICA
Publication statusPublished - 2011
EventXII Conferenza Nazionale di Sanità Pubblica ROMA 12-15 ottobre 2011 LA SANITÀ PUBBLICA TRA GLOBALIZZAZIONE, NUOVE ESIGENZE DI SALUTE E SOSTENIBILITÀ ECONOMICA: LA SFIDA DELL’ INTEGRAZIONE - Roma
Duration: 12 Oct 201115 Oct 2011

Keywords

  • antibioticoresistenza
  • studio cross sectional

Fingerprint

Dive into the research topics of '[Autom. eng. transl.] Comparison of the antibiotic resistance of Pseudomonas aeruginosa strains and non-fermenting Gram negative bacteria: a cross sectional study conducted on isolates from environmental matrices in a University Hospital of Rome'. Together they form a unique fingerprint.

Cite this