Comparison of temperature and moisture requirements for sporulation of Aspergillus flavus sclerotia on natural and artificial substrates

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

A key step in the infection cycle by Aspergillus flavus in maize is sporulation of sclerotia present in soil or in crop debris. However, little information is available on this critical and important phase. This study included experiments on artificial (CZ agar) and natural (maize stalks) substrates under different conditions of temperature (T; from 5 to 45°C) and water activity (aw; from 0.50 to 0.99) levels to quantify sporulation from sclerotia. The mean numbers of spores was higher on defined nutritional medium in vitro on CZ agar than on maize stalks (4.5 x 106 versus 4.2 x 104) with production initiated after 6 and 24 hours, respectively. Surprisingly, optimal temperature was found at 30-35°C for CZ agar (9.23 x 106 spores/sclerotium) and at 20-25°C for maize stalks (6.26 x 104 spores/sclerotium). Water stress imposition only reduced sporulation at ≤ 0.90 aw. With more available water no significant differences were found between 0.90-0.99 aw. This type of data is critical in the development of a mechanistic model to predict the infection cycle of A. flavus in maize in relation to meteorological conditions.
Original languageEnglish
Pages (from-to)637-642
Number of pages6
JournalFungal Biology
Volume116
DOIs
Publication statusPublished - 2012

Keywords

  • Aspergillus flavus
  • aw
  • maize
  • sporulation
  • temperature

Fingerprint

Dive into the research topics of 'Comparison of temperature and moisture requirements for sporulation of Aspergillus flavus sclerotia on natural and artificial substrates'. Together they form a unique fingerprint.

Cite this