[Comparison of conventional culture methods and quantitative real-time PCR methods for the detection of Legionella pneumophila in water samples in a large University teaching hospital in Rome, Italy]

Translated title of the contribution: [Autom. eng. transl.] [Comparison of conventional culture methods and quantitative real-time PCR methods for the detection of Legionella pneumophila in water samples in a large University teaching hospital in Rome, Italy]

Stefania Boccia, Patrizia Laurenti, Stefania Bruno, Gianfranco Damiani, Umberto Moscato, Brunella Posteraro, Gualtiero Ricciardi, Gianluigi Quaranta, Emanuele Leoncini, Sara Vincenti, Dario Arzani, Filippo Berloco, Federica Boninti, Fabrizio Celani, Paolo Di Giannantonio, Romina Sezzatini, Alessia Vecchioni, Malgorzata Wachocka, Maria Giovanna Ficarra

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aims of this study were to identify the best threshold value for the real-time PCR method in detecting the presence of Legionella pneumophila in water samples, and to evaluate the prognostic significance of negative results obtained with the molecular method. From 2011 to 2014, 77 water samples were collected from hospital wards of a large University teaching hospital in Rome (Italy) and screened for L.pneumophila by the standard culture method and by real-time PCR. The high sensitivity and negative predictive value of real-time PCR make this method suitable as a quick screening tool to exclude the presence of L. pneumophila in water samples in the hospital setting.
Translated title of the contribution[Autom. eng. transl.] [Comparison of conventional culture methods and quantitative real-time PCR methods for the detection of Legionella pneumophila in water samples in a large University teaching hospital in Rome, Italy]
Original languageItalian
Pages (from-to)569-576
Number of pages8
JournalIGIENE E SANITÀ PUBBLICA
Volume71
Publication statusPublished - 2015

Keywords

  • Legionella pneumophila
  • quantitative real-time PCR

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