Abstract
A liquid chromatography method coupled with an Orbitrap analyser was used to detect nisin A and its variants in partially skimmed and raw cow milk. A suspect screening method enabled the identification of F, Q and Z variants. Adequate linearity was achieved (r(2) = 0.9998-1 up to 250 mu g/L) with a limit of quantitation for nisin A of 2.5 mu g/L. Method accuracy (at 2.5, 25 and 250 mu g/L) in milk ranged from 94 to 98% and precision in the range 0.4-1.9%. Nisin Z was abundant in partially skimmed samples. The method was suitable for determining these bacteriocins in cow milk.
| Original language | English |
|---|---|
| Pages (from-to) | 610-614 |
| Number of pages | 5 |
| Journal | International Journal of Dairy Technology |
| Volume | 77 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2024 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
All Science Journal Classification (ASJC) codes
- Food Science
- Bioengineering
- Process Chemistry and Technology
Keywords
- Bacteriocins
- High-resolution mass spectrometry
- Nisin variants
- Suspect screening
- Ultra-high-performance liquid chromatography
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