Characterization of the Protein Components of Matrix Stones Sheds Light on S100-A8 and S100-A9 Relevance in the Inflammatory Pathogenesis of These Rare Renal Calculi

Federica Iavarone, Pietro Manuel Ferraro, Claudia Martelli, Valeria Marzano, Liling Huang, Claudia Desiderio, Irene Messana, Massimo Castagnola, Giovanni Gambaro, Paolo Beltrami, Filiberto Zattoni, Noor Buchholz, Giorgia Locci, Gavino Faa

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

PURPOSE: Among the different types of kidney stones, matrix stones are uncommon urinary calculi composed of a soft, pliable, amorphous substance with little crystalline content. To gain insight into the pathogenesis we investigated the protein component by analyzing the proteomic profiles of surgically removed matrix stones. MATERIALS AND METHODS: A total of 5 stones were harvested from 4 patients who underwent surgery for medical reasons at 3 clinical centers during a 7-year period. Matrix stone proteome characterization was performed by mass spectrometry based techniques using an integrated top-down/bottom-up proteomic platform. RESULTS: We identified 142 nonredundant proteins and peptides across all samples. Neutrophil defensin 1, and proteins S100-A8 and S100-A9 were the main components of these renal calculi. CONCLUSIONS: The abundance of identified inflammatory molecules points to an inflammatory process as the event that initializes soft calculi formation rather than as a consequence of such formation. The post-translational oxidative changes in S100-A8 and A9, and the presence of thymosin β-4, granulins and ubiquitin also suggest the intervention of host defenses through a superimposed, vigorous counter inflammatory process. The post-translational changes seen in the proteins and peptides, and the known self-assembling capability of S100-A8 and S100-A9 probably explain the gelatinous consistency of these stones.
Original languageEnglish
Pages (from-to)911-918
Number of pages8
JournalTHE JOURNAL OF UROLOGY
Volume196
DOIs
Publication statusPublished - 2016

Keywords

  • inflammation
  • kidney calculi
  • proteomics

Fingerprint

Dive into the research topics of 'Characterization of the Protein Components of Matrix Stones Sheds Light on S100-A8 and S100-A9 Relevance in the Inflammatory Pathogenesis of These Rare Renal Calculi'. Together they form a unique fingerprint.

Cite this