Characterization of the human salivary basic proline-rich protein complex by a proteomic approach

Massimo Cordaro, Irene Messana, Tiziana Cabras, Rosanna Inzitari, Alessandro Lupi, Cecilia Zuppi, Chiara Olmi, Maria Benedetta Fadda, Bruno Giardina, Massimo Castagnola

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88 Citations (Scopus)

Abstract

Thirteen samples of human normal whole saliva were analyzed by RP-HPLC-ESI-MS and MALDI-TOF-MS to investigate the basic proline-rich protein complex. Between known basic-PRPs the P-B, P-C (or IB-8b), P-D (or IB-5), P-E (or IB-9), P-F (or IB-8), P-H (or IB-4), IB-6,II-2, IB-1, and IB-8a glucosylated were identified, whereas the II-1, IB-7, PA, and D1-A peptides were not detected. Some detected masses not attributable to known basic-PRPs were putatively ascribed to II-2 and IB-1 nonphosphorylated, II-2 and IB-1 missing the C-terminal arginine residue, and the 1-62 fragment of IB-6, named P-J peptide. A correlation matrix analysis revealed a cluster of correlation among all the basic PRF's (apart from the P-B peptide) which is in agreement with their common parotid origin.
Original languageEnglish
Pages (from-to)792-800
Number of pages9
JournalJournal of Proteome Research
Publication statusPublished - 2004

Keywords

  • PEPTIDE
  • SALIVARY

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