Abstract

Ascorbic acid, isoascorbic acid and dehydroascorbic acid inhibit bovine kidney alkaline phosphatase activity. Ascorbic acid free radicals seem not to be involved. Dialysis does not make the inactivation reversible. A competitive mechanism can be inferred from experiments with phosphate and substrates, which block the activity decay. The influence of temperature, pH, other inhibitors and tertiary structure modifications on the inactivation process is also investigated.
Original languageEnglish
Pages (from-to)343-346
Number of pages4
JournalBIOCHIMICA ET BIOPHYSICA ACTA
Volume789
Publication statusPublished - 1984

Keywords

  • Alkaline Phosphatase
  • Animals
  • Ascorbic Acid
  • Binding, Competitive
  • Cattle
  • Dehydroascorbic Acid
  • Hydrogen-Ion Concentration
  • Kidney
  • Structure-Activity Relationship
  • Temperature

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