Autoimmune hemolytic anemia with gel-based immunohematology tests

Raffaele Landolfi, Marco Lai, Giuseppe Leone

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We used gel centrifugation tests (GCTs) to analyze the relationship between the diagnosis and immunohematology tests used for autoimmune hemolytic anemia (AIHA). The study included 588 samples positive for the direct antiglobulin test (DAT). Of these, 52 were from patients diagnosed with AIHA. Immunoglobulin (Ig) class, IgG1, IgG3, and complement were measured. DAT strength had the strongest correlation with AIHA diagnosis (odds ratio [OR], 23), followed by anti-IgG titer 300 (OR, 8.4), anti-IgG titer 1,000 (OR, 10.5), and C3d agglutination strength (OR, 1.7). Decision tree analysis revealed that DAT strength and anti-IgG titer higher than 100 were the best predictors of AIHA. Multidimensional scanning analysis found a high grade of similarity among DAT strength, anti-IgG titer, and IgG strength in the AIHA samples. This observation was not detected in DAT-positive samples from patients without AIHA. DAT strength remained the best diagnostic indicator for AIHA and had the strongest association with AIHA compared with other commercially available immunohematology tests. The other tests, despite good correlation with AIHA diagnosis, did not add useful information
Original languageEnglish
Pages (from-to)457-463
Number of pages7
JournalAmerican Journal of Clinical Pathology
DOIs
Publication statusPublished - 2013

Keywords

  • hemolytic anemia

Fingerprint

Dive into the research topics of 'Autoimmune hemolytic anemia with gel-based immunohematology tests'. Together they form a unique fingerprint.

Cite this