ASSESSMENT OF ANGIOGENESIS BY CD105 AND NESTIN EXPRESSION IN PERITUMOR TISSUE OF GLIOBLASTOMA

Maria Concetta Geloso, Gigliola Sica, Gina Lama, Carmelo Anile, Giuseppe La Torre, Pasquale De Bonis, Giulio Maira, Libero Lauriola, Annunziato Mangiola, Meena Jhanwar Uniyal

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Angiogenesis in the peritumor tissue of glioblastoma (GBM) is still an open field of research. This study investigates neovascularization in the tumor surrounding areas by examining CD105 and nestin expression along with microvessel density (MVD) with the aim of establishing their possible prognostic significance. Angiogenesis was also confirmed by investigating, in vessel walls, the presence of pericytes, which are multipotent stem cells, expressing α-smooth muscle actin (α-SMA). In our study, including 40 GBM patients, tissue samples were obtained from tumors (first area) and white matter at a distance <1 cm (second area) and between 1 and 3.5 cm (third area) from the tumor margin. CD105 and nestin were detected by immunohistochemistry in hyperplastic endothelium of GBM and peritumor tissue, and occasionally coexpressed or colocalized. Pericytes encircling hyperplastic endothelium were evident in all three areas. Univariate analysis revealed that patients with a CD105-MVD value ≥8 in the third area have a significantly shorter survival time and Cox analysis indicated an about 3.5-fold increase in death risk in the same patients. These results demonstrate that a tumor neoangiogenesis occurs in GBM peritumor tissue with intimate involvement of pericytes. CD105-MVD in the area located at a greater distance from the tumor margin carries prognostic significance
Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalInternational Journal of Oncology
Volume38
Publication statusPublished - 2011

Keywords

  • ANGIOGENESIS
  • CD105
  • GLIOBLASTOMA
  • MICROVESSEL DENSITY
  • NESTIN
  • PERITUMOR TISSUE

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