Abstract
alpha-Bungarotoxin (alpha-BuTX) has been used as a marker for studying the production of alpha-bungarotoxin-acetylcholine receptors (alpha-BuTX-AChRs) in explants of chick embryo sympathetic ganglia cultured in vitro. New alpha-BuTX-AChRs appear rapidly in the explants after blocking of the pre-existent ones with the toxin (40% of the total receptors at 3 h). There is a portion of alpha-BuTX-AChRs in the explants which for a short time is not accessible to the toxin. This portion constitutes the precursor pool of receptors and represents 18% of the total. The precursor pool of receptors supplies the neurons with new receptors for 1-2 h in the absence of protein synthesis. The appearance of new receptors from the precursor pool is an energy-dependent process.
| Original language | English |
|---|---|
| Pages (from-to) | 131-141 |
| Number of pages | 11 |
| Journal | Brain Research |
| Volume | 161 |
| Publication status | Published - 1979 |
| Externally published | Yes |
Keywords
- Acetylcholine
- Animals
- Autoradiography
- Binding, Competitive
- Bungarotoxins
- Cell Differentiation
- Chick Embryo
- Ganglia, Autonomic
- Models, Neurological
- Receptors, Cholinergic
- Tubocurarine