An integrated superhydrophobic-plasmonic biosensor for mid-infrared protein detection at the femtomole level

Adele De Ninno; Gabriele Ciasca; Annamaria Gerardino; Eugenio Calandrini; Massimiliano Papi; Marco De Spirito; Alessandro Nucara; Michele Ortolani; Luca Businaro; Leonetta Baldassarre

doi:10.1039/c4cp05023a

An integrated superhydrophobic-plasmonic biosensor for mid-infrared protein detection at the femtomole level

Adele De Ninno, Gabriele Ciasca, Annamaria Gerardino, Eugenio Calandrini, Massimiliano Papi, Marco De Spirito, Alessandro Nucara, Michele Ortolani, Luca Businaro, Leonetta Baldassarre

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17 Citations (Scopus)

Abstract

In this work we present an integrated biosensor that enables FTIR (Fourier Transform-Infrared) detection of analytes contained in diluted solutions. The fabricated nanosensor allows for the detection of proteins through the identification of the fine structure of their amide I and II bands, up to the nanomolar concentration range. We exploited two distinct effects to enhance the sensitivity: (i) the concentration effect due to the presence of the superhydrophobic surface that conveys molecules dispersed in solution directly inside the focus of a FTIR spectromicroscope; (ii) the plasmonic resonance of the nanoantenna array that provides electromagnetic field enhancement in the amide I and II spectral region (1500–1700 cm−1). We demonstrate the detection of ferritin in the nanomolar concentration range, a blood protein that is usually available in small amounts in typical blood samples.

Original language	English
Pages (from-to)	21337-21342
Number of pages	8
Journal	Physical Chemistry Chemical Physics
Volume	17
DOIs	https://doi.org/10.1039/c4cp05023a
Publication status	Published - 2015

Keywords

Surface Enhanced Spectroscopy

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10.1039/c4cp05023a

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title = "An integrated superhydrophobic-plasmonic biosensor for mid-infrared protein detection at the femtomole level",

abstract = "In this work we present an integrated biosensor that enables FTIR (Fourier Transform-Infrared) detection of analytes contained in diluted solutions. The fabricated nanosensor allows for the detection of proteins through the identification of the fine structure of their amide I and II bands, up to the nanomolar concentration range. We exploited two distinct effects to enhance the sensitivity: (i) the concentration effect due to the presence of the superhydrophobic surface that conveys molecules dispersed in solution directly inside the focus of a FTIR spectromicroscope; (ii) the plasmonic resonance of the nanoantenna array that provides electromagnetic field enhancement in the amide I and II spectral region (1500–1700 cm−1). We demonstrate the detection of ferritin in the nanomolar concentration range, a blood protein that is usually available in small amounts in typical blood samples.",

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author = "{De Ninno}, Adele and Gabriele Ciasca and Annamaria Gerardino and Eugenio Calandrini and Massimiliano Papi and {De Spirito}, Marco and Alessandro Nucara and Michele Ortolani and Luca Businaro and Leonetta Baldassarre",

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doi = "10.1039/c4cp05023a",

language = "English",

volume = "17",

pages = "21337--21342",

journal = "Physical Chemistry Chemical Physics",

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publisher = "[London, England] : Royal Society of Chemistry, 1999-",

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T1 - An integrated superhydrophobic-plasmonic biosensor for mid-infrared protein detection at the femtomole level

AU - De Ninno, Adele

AU - Ciasca, Gabriele

AU - Gerardino, Annamaria

AU - Calandrini, Eugenio

AU - Papi, Massimiliano

AU - De Spirito, Marco

AU - Nucara, Alessandro

AU - Ortolani, Michele

AU - Businaro, Luca

AU - Baldassarre, Leonetta

PY - 2015

Y1 - 2015

N2 - In this work we present an integrated biosensor that enables FTIR (Fourier Transform-Infrared) detection of analytes contained in diluted solutions. The fabricated nanosensor allows for the detection of proteins through the identification of the fine structure of their amide I and II bands, up to the nanomolar concentration range. We exploited two distinct effects to enhance the sensitivity: (i) the concentration effect due to the presence of the superhydrophobic surface that conveys molecules dispersed in solution directly inside the focus of a FTIR spectromicroscope; (ii) the plasmonic resonance of the nanoantenna array that provides electromagnetic field enhancement in the amide I and II spectral region (1500–1700 cm−1). We demonstrate the detection of ferritin in the nanomolar concentration range, a blood protein that is usually available in small amounts in typical blood samples.

AB - In this work we present an integrated biosensor that enables FTIR (Fourier Transform-Infrared) detection of analytes contained in diluted solutions. The fabricated nanosensor allows for the detection of proteins through the identification of the fine structure of their amide I and II bands, up to the nanomolar concentration range. We exploited two distinct effects to enhance the sensitivity: (i) the concentration effect due to the presence of the superhydrophobic surface that conveys molecules dispersed in solution directly inside the focus of a FTIR spectromicroscope; (ii) the plasmonic resonance of the nanoantenna array that provides electromagnetic field enhancement in the amide I and II spectral region (1500–1700 cm−1). We demonstrate the detection of ferritin in the nanomolar concentration range, a blood protein that is usually available in small amounts in typical blood samples.

KW - Surface Enhanced Spectroscopy

UR - http://hdl.handle.net/10807/66190

U2 - 10.1039/c4cp05023a

DO - 10.1039/c4cp05023a

M3 - Article

SN - 1463-9076

VL - 17

SP - 21337

EP - 21342

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

ER -

An integrated superhydrophobic-plasmonic biosensor for mid-infrared protein detection at the femtomole level

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Keywords

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