Amino Acid Profiles in Older Adults with Frailty: Secondary Analysis from MetaboFrail and BIOSPHERE Studies

Riccardo Calvani; Anna Picca; Leocadio Rodríguez-Mañas; Matteo Tosato; Hélio José Coelho-Júnior; Alessandra Biancolillo; Olga Laosa; Jacopo Gervasoni; Aniello Primiano; Lavinia Santucci; OTTAVIA GIAMPAOLI; Isabelle Bourdel-Marchasson; Sophie C. Regueme; Alan J. Sinclair; Andrea Urbani; Francesco Landi; Giovanni Gambassi; Federico Marini; Emanuele Marzetti

doi:10.3390/metabo13040542

Amino Acid Profiles in Older Adults with Frailty: Secondary Analysis from MetaboFrail and BIOSPHERE Studies

Riccardo Calvani^*
, Anna Picca
, Leocadio Rodríguez-Mañas
, Matteo Tosato
, Hélio José Coelho-Júnior
, Alessandra Biancolillo
, Olga Laosa
, Jacopo Gervasoni
, Aniello Primiano
, Lavinia Santucci
, OTTAVIA GIAMPAOLI
, Isabelle Bourdel-Marchasson
, Sophie C. Regueme
, Alan J. Sinclair
, Andrea Urbani
, Francesco Landi
, Giovanni Gambassi
, Federico Marini
, Emanuele Marzetti

^*Corresponding author

Università LUM
University of L'Aquila
Hospital Universitario de Getafe
CHU Hôpitaux de Bordeaux
King's College London
University of Rome La Sapienza

Research output: Contribution to journal › Article

Abstract

An altered amino acid metabolism has been described in frail older adults which may contribute to muscle loss and functional decline associated with frailty. In the present investigation, we compared circulating amino acid profiles of older adults with physical frailty and sarcopenia (PF&S, n = 94), frail/pre-frail older adults with type 2 diabetes mellitus (F-T2DM, n = 66), and robust non-diabetic controls (n = 40). Partial least squares discriminant analysis (PLS-DA) models were built to define the amino acid signatures associated with the different frailty phenotypes. PLS-DA allowed correct classification of participants with 78.2 ± 1.9% accuracy. Older adults with F-T2DM showed an amino acid profile characterized by higher levels of 3-methylhistidine, alanine, arginine, ethanolamine, and glutamic acid. PF&S and control participants were discriminated based on serum concentrations of aminoadipic acid, aspartate, citrulline, cystine, taurine, and tryptophan. These findings suggest that different types of frailty may be characterized by distinct metabolic perturbations. Amino acid profiling may therefore serve as a valuable tool for frailty biomarker discovery.

Original language	English
Pages (from-to)	1-15
Number of pages	15
Journal	Metabolites
Volume	13
Issue number	4
DOIs	https://doi.org/10.3390/metabo13040542
Publication status	Published - 2023

All Science Journal Classification (ASJC) codes

Endocrinology, Diabetes and Metabolism
Biochemistry
Molecular Biology

Keywords

aging
geroscience
metabolic profiling
metabolism
metabolomics
muscle wasting
physical function
protein
sarcopenia
systems biology

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10.3390/metabo13040542

Cite this

Calvani, R., Picca, A., Rodríguez-Mañas, L., Tosato, M., Coelho-Júnior, H. J., Biancolillo, A., Laosa, O., Gervasoni, J., Primiano, A., Santucci, L., GIAMPAOLI, OTTAVIA., Bourdel-Marchasson, I., Regueme, S. C., Sinclair, A. J., Urbani, A., Landi, F., Gambassi, G., Marini, F., & Marzetti, E. (2023). Amino Acid Profiles in Older Adults with Frailty: Secondary Analysis from MetaboFrail and BIOSPHERE Studies. Metabolites, 13(4), 1-15. https://doi.org/10.3390/metabo13040542

@article{cfc958c5a15c4bd1a366d1f482213757,

title = "Amino Acid Profiles in Older Adults with Frailty: Secondary Analysis from MetaboFrail and BIOSPHERE Studies",

abstract = "An altered amino acid metabolism has been described in frail older adults which may contribute to muscle loss and functional decline associated with frailty. In the present investigation, we compared circulating amino acid profiles of older adults with physical frailty and sarcopenia (PF\&S, n = 94), frail/pre-frail older adults with type 2 diabetes mellitus (F-T2DM, n = 66), and robust non-diabetic controls (n = 40). Partial least squares discriminant analysis (PLS-DA) models were built to define the amino acid signatures associated with the different frailty phenotypes. PLS-DA allowed correct classification of participants with 78.2 ± 1.9\% accuracy. Older adults with F-T2DM showed an amino acid profile characterized by higher levels of 3-methylhistidine, alanine, arginine, ethanolamine, and glutamic acid. PF\&S and control participants were discriminated based on serum concentrations of aminoadipic acid, aspartate, citrulline, cystine, taurine, and tryptophan. These findings suggest that different types of frailty may be characterized by distinct metabolic perturbations. Amino acid profiling may therefore serve as a valuable tool for frailty biomarker discovery.",

keywords = "aging, geroscience, metabolic profiling, metabolism, metabolomics, muscle wasting, physical function, protein, sarcopenia, systems biology, aging, geroscience, metabolic profiling, metabolism, metabolomics, muscle wasting, physical function, protein, sarcopenia, systems biology",

author = "Riccardo Calvani and Anna Picca and Leocadio Rodr{\'i}guez-Ma{\~n}as and Matteo Tosato and Coelho-J{\'u}nior, \{H{\'e}lio Jos{\'e}\} and Alessandra Biancolillo and Olga Laosa and Jacopo Gervasoni and Aniello Primiano and Lavinia Santucci and OTTAVIA GIAMPAOLI and Isabelle Bourdel-Marchasson and Regueme, \{Sophie C.\} and Sinclair, \{Alan J.\} and Andrea Urbani and Francesco Landi and Giovanni Gambassi and Federico Marini and Emanuele Marzetti",

year = "2023",

doi = "10.3390/metabo13040542",

language = "English",

volume = "13",

pages = "1--15",

journal = "Metabolites",

issn = "2218-1989",

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Calvani, R, Picca, A, Rodríguez-Mañas, L, Tosato, M, Coelho-Júnior, HJ, Biancolillo, A, Laosa, O, Gervasoni, J, Primiano, A, Santucci, L, GIAMPAOLI, OTTAVIA, Bourdel-Marchasson, I, Regueme, SC, Sinclair, AJ, Urbani, A , Landi, F , Gambassi, G, Marini, F & Marzetti, E 2023, 'Amino Acid Profiles in Older Adults with Frailty: Secondary Analysis from MetaboFrail and BIOSPHERE Studies', Metabolites, vol. 13, no. 4, pp. 1-15. https://doi.org/10.3390/metabo13040542

TY - JOUR

T1 - Amino Acid Profiles in Older Adults with Frailty: Secondary Analysis from MetaboFrail and BIOSPHERE Studies

AU - Calvani, Riccardo

AU - Picca, Anna

AU - Rodríguez-Mañas, Leocadio

AU - Tosato, Matteo

AU - Coelho-Júnior, Hélio José

AU - Biancolillo, Alessandra

AU - Laosa, Olga

AU - Gervasoni, Jacopo

AU - Primiano, Aniello

AU - Santucci, Lavinia

AU - GIAMPAOLI, OTTAVIA

AU - Bourdel-Marchasson, Isabelle

AU - Regueme, Sophie C.

AU - Sinclair, Alan J.

AU - Urbani, Andrea

AU - Landi, Francesco

AU - Gambassi, Giovanni

AU - Marini, Federico

AU - Marzetti, Emanuele

PY - 2023

Y1 - 2023

N2 - An altered amino acid metabolism has been described in frail older adults which may contribute to muscle loss and functional decline associated with frailty. In the present investigation, we compared circulating amino acid profiles of older adults with physical frailty and sarcopenia (PF&S, n = 94), frail/pre-frail older adults with type 2 diabetes mellitus (F-T2DM, n = 66), and robust non-diabetic controls (n = 40). Partial least squares discriminant analysis (PLS-DA) models were built to define the amino acid signatures associated with the different frailty phenotypes. PLS-DA allowed correct classification of participants with 78.2 ± 1.9% accuracy. Older adults with F-T2DM showed an amino acid profile characterized by higher levels of 3-methylhistidine, alanine, arginine, ethanolamine, and glutamic acid. PF&S and control participants were discriminated based on serum concentrations of aminoadipic acid, aspartate, citrulline, cystine, taurine, and tryptophan. These findings suggest that different types of frailty may be characterized by distinct metabolic perturbations. Amino acid profiling may therefore serve as a valuable tool for frailty biomarker discovery.

AB - An altered amino acid metabolism has been described in frail older adults which may contribute to muscle loss and functional decline associated with frailty. In the present investigation, we compared circulating amino acid profiles of older adults with physical frailty and sarcopenia (PF&S, n = 94), frail/pre-frail older adults with type 2 diabetes mellitus (F-T2DM, n = 66), and robust non-diabetic controls (n = 40). Partial least squares discriminant analysis (PLS-DA) models were built to define the amino acid signatures associated with the different frailty phenotypes. PLS-DA allowed correct classification of participants with 78.2 ± 1.9% accuracy. Older adults with F-T2DM showed an amino acid profile characterized by higher levels of 3-methylhistidine, alanine, arginine, ethanolamine, and glutamic acid. PF&S and control participants were discriminated based on serum concentrations of aminoadipic acid, aspartate, citrulline, cystine, taurine, and tryptophan. These findings suggest that different types of frailty may be characterized by distinct metabolic perturbations. Amino acid profiling may therefore serve as a valuable tool for frailty biomarker discovery.

KW - aging

KW - geroscience

KW - metabolic profiling

KW - metabolism

KW - metabolomics

KW - muscle wasting

KW - physical function

KW - protein

KW - sarcopenia

KW - systems biology

KW - aging

KW - geroscience

KW - metabolic profiling

KW - metabolism

KW - metabolomics

KW - muscle wasting

KW - physical function

KW - protein

KW - sarcopenia

KW - systems biology

UR - https://publicatt.unicatt.it/handle/10807/234097

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U2 - 10.3390/metabo13040542

DO - 10.3390/metabo13040542

M3 - Article

SN - 2218-1989

VL - 13

SP - 1

EP - 15

JO - Metabolites

JF - Metabolites

IS - 4

ER -

Amino Acid Profiles in Older Adults with Frailty: Secondary Analysis from MetaboFrail and BIOSPHERE Studies

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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