Alkaline-phosphatase-based nanostructure assemblies for electrochemical detection of microRNAs

D. Voccia; Diego Voccia; F. Bettazzi; G. Baydemir; I. Palchetti

doi:10.1166/jnn.2015.10201

Alkaline-phosphatase-based nanostructure assemblies for electrochemical detection of microRNAs

D. Voccia, Diego Voccia, F. Bettazzi, G. Baydemir, I. Palchetti

University of Florence

Research output: Contribution to journal › Article

15 Citations (Scopus)

Abstract

Different nanoarchitectures, rich in enzyme labels, are herein investigated for signal amplification in the electrochemical detection of nucleic acids and in particular of microRNAs. Dendritic amplification, accomplished by the use of streptavidin and biotinylated alkaline phosphatase, and enzymedecorated liposomes are used as labels to amplify the microRNA-sensing, by their association to the probe-microRNA hybrid generated onto a gold transducer. Differential pulse voltammetry and faradaic impedance spectroscopy were employed to characterize these different amplification routes.

Original language	English
Pages (from-to)	3378-3384
Number of pages	7
Journal	Journal of Nanoscience and Nanotechnology
Volume	15
DOIs	https://doi.org/10.1166/jnn.2015.10201
Publication status	Published - 2015

Keywords

Alkaline Phosphatase
Biosensing Techniques
Biotin
Biotinylated alkaline phosphatase
Electrochemical Techniques
Enzyme-Decorated liposome
Liposomes
MicroRNAs
Nanostructures
Streptavidin
miRNA-221

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10.1166/jnn.2015.10201

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title = "Alkaline-phosphatase-based nanostructure assemblies for electrochemical detection of microRNAs",

abstract = "Different nanoarchitectures, rich in enzyme labels, are herein investigated for signal amplification in the electrochemical detection of nucleic acids and in particular of microRNAs. Dendritic amplification, accomplished by the use of streptavidin and biotinylated alkaline phosphatase, and enzymedecorated liposomes are used as labels to amplify the microRNA-sensing, by their association to the probe-microRNA hybrid generated onto a gold transducer. Differential pulse voltammetry and faradaic impedance spectroscopy were employed to characterize these different amplification routes.",

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author = "D. Voccia and Diego Voccia and F. Bettazzi and G. Baydemir and I. Palchetti",

year = "2015",

doi = "10.1166/jnn.2015.10201",

language = "English",

volume = "15",

pages = "3378--3384",

journal = "Journal of Nanoscience and Nanotechnology",

issn = "1533-4880",

publisher = "American Scientific Publishers:25650 North Lewis Way:Stevenson Ranch, CA 91381:(661)254-0807, EMAIL: [email protected], INTERNET: http://www.aspbs.com, Fax: (661)254-1207",

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TY - JOUR

T1 - Alkaline-phosphatase-based nanostructure assemblies for electrochemical detection of microRNAs

AU - Voccia, D.

AU - Voccia, Diego

AU - Bettazzi, F.

AU - Baydemir, G.

AU - Palchetti, I.

PY - 2015

Y1 - 2015

N2 - Different nanoarchitectures, rich in enzyme labels, are herein investigated for signal amplification in the electrochemical detection of nucleic acids and in particular of microRNAs. Dendritic amplification, accomplished by the use of streptavidin and biotinylated alkaline phosphatase, and enzymedecorated liposomes are used as labels to amplify the microRNA-sensing, by their association to the probe-microRNA hybrid generated onto a gold transducer. Differential pulse voltammetry and faradaic impedance spectroscopy were employed to characterize these different amplification routes.

AB - Different nanoarchitectures, rich in enzyme labels, are herein investigated for signal amplification in the electrochemical detection of nucleic acids and in particular of microRNAs. Dendritic amplification, accomplished by the use of streptavidin and biotinylated alkaline phosphatase, and enzymedecorated liposomes are used as labels to amplify the microRNA-sensing, by their association to the probe-microRNA hybrid generated onto a gold transducer. Differential pulse voltammetry and faradaic impedance spectroscopy were employed to characterize these different amplification routes.

KW - Alkaline Phosphatase

KW - Biosensing Techniques

KW - Biotin

KW - Biotinylated alkaline phosphatase

KW - Electrochemical Techniques

KW - Enzyme-Decorated liposome

KW - Liposomes

KW - MicroRNAs

KW - Nanostructures

KW - Streptavidin

KW - miRNA-221

KW - Alkaline Phosphatase

KW - Biosensing Techniques

KW - Biotin

KW - Biotinylated alkaline phosphatase

KW - Electrochemical Techniques

KW - Enzyme-Decorated liposome

KW - Liposomes

KW - MicroRNAs

KW - Nanostructures

KW - Streptavidin

KW - miRNA-221

UR - http://hdl.handle.net/10807/155918

U2 - 10.1166/jnn.2015.10201

DO - 10.1166/jnn.2015.10201

M3 - Article

SN - 1533-4880

VL - 15

SP - 3378

EP - 3384

JO - Journal of Nanoscience and Nanotechnology

JF - Journal of Nanoscience and Nanotechnology

ER -

Alkaline-phosphatase-based nanostructure assemblies for electrochemical detection of microRNAs

Abstract

Keywords

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