Alkaline-phosphatase-based nanostructure assemblies for electrochemical detection of microRNAs

Diego Voccia, D. Voccia, F. Bettazzi, G. Baydemir, I. Palchetti

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Different nanoarchitectures, rich in enzyme labels, are herein investigated for signal amplification in the electrochemical detection of nucleic acids and in particular of microRNAs. Dendritic amplification, accomplished by the use of streptavidin and biotinylated alkaline phosphatase, and enzymedecorated liposomes are used as labels to amplify the microRNA-sensing, by their association to the probe-microRNA hybrid generated onto a gold transducer. Differential pulse voltammetry and faradaic impedance spectroscopy were employed to characterize these different amplification routes.
Original languageEnglish
Pages (from-to)3378-3384
Number of pages7
JournalJournal of Nanoscience and Nanotechnology
Volume15
DOIs
Publication statusPublished - 2015

Keywords

  • Alkaline Phosphatase
  • Biosensing Techniques
  • Biotin
  • Biotinylated alkaline phosphatase
  • Electrochemical Techniques
  • Enzyme-Decorated liposome
  • Liposomes
  • MicroRNAs
  • Nanostructures
  • Streptavidin
  • miRNA-221

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