A kinetic approach to the evaluation of alkaline phosphatase inactivation by urea

Alvaro Mordente, Elisabetta Meucci, G. E. Martorana, A. Castelli

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A kinetic approach is described which enables the measurement of the enzyme inactivation rate constant during the reaction course. A mathematical analysis is presented and it is shown that a time-dependent step may be postulated to exist. Reaction kinetics follow an exponential rule with time as the independent variable and enzymatic activity as the dependent variable. A simple procedure of graphical analysis is reported and the influence on the inactivation rate constant of various conditions (temperature and inhibitor concentration) is evaluated. The method is illustrated by an experimental model: the inactivation of bovine kidney alkaline phosphatase by urea.
Original languageEnglish
Pages (from-to)223-230
Number of pages8
JournalItalian Journal of Biochemistry
Volume32
Publication statusPublished - 1983

Keywords

  • Alkaline Phosphatase
  • Animals
  • Cattle
  • Kidney
  • Kinetics
  • Urea

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