1H NMR metabolite fingerprinting as a new tool for body fluid identification in forensic science

Fabio De Giorgio, Ernesto D'Aloja, Paola Scano, Emanuela Locci, Antonio Noto, Gabriele Navarra, Federica Murgia, Milena Lussu, Luigi Barberini, Luigi Atzori, Maria Francesca Rosa

Research output: Contribution to journalArticle

11 Citations (Scopus)


In this feasibility study, we propose, for the first time, (1)H NMR spectroscopy coupled with mathematical strategies as a valid tool for body fluid (BF) trace identification in forensic science. In order to assess the ability of this approach to identify traces composed either by a single or by two different BFs, samples of blood, urine, saliva, and semen were collected from different donors, and binary mixtures were prepared. (1)H NMR analyses were carried out for all samples. Spectral data of the whole set were firstly submitted to unsupervised principal component analysis (PCA); it showed that samples of the same BF cluster well on the basis of their characterizing molecular components and that mixtures exhibit intermediate characteristics among BF typologies. Furthermore, samples were divided into a training set and a test set. An average NMR spectral profile for each typology of BF was obtained from the training set and validated as representative of each BF class. Finally, a fitting procedure, based on a system of linear equations with the four obtained average spectral profiles, was applied to the test set and the mixture samples; it showed that BFs can be unambiguously identified, even as components of a mixture. The successful use of this mathematical procedure has the advantage, in forensics, of overcoming bias due to the analyst's personal judgment. We therefore propose this combined approach as a valid, fast, and non-destructive tool for addressing the challenges in the identification of composite traces in forensics.
Original languageEnglish
Pages (from-to)454-462
Number of pages9
JournalMagnetic resonance in chemistry : MRC
Publication statusPublished - 2013


  • 1H
  • NMR
  • body fluids traces
  • forensic science
  • metabolite profile


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